Recombinant Human Golgi SNAP receptor complex member 1 (GOSR1)
Description
Protein Structure
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Gene ID: 9527 (Human), encoding a 250-amino acid protein with a molecular weight of ~28 kDa (unmodified) .
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Domains: Contains a single-pass transmembrane domain (type IV) critical for Golgi membrane localization .
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Post-Translational Modifications: Predicted phosphorylation and glycosylation sites influence its interaction with COPI/II vesicles .
Functional Role
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Vesicle Trafficking: Facilitates ER-to-Golgi and intra-Golgi transport by forming SNARE complexes with partners like STX5, BET1L, and YKT6 .
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Oxidative Stress Response: Regulates reactive oxygen species (ROS) levels in neuronal cells via p38 MAPK inhibition under glutathione-depleted conditions .
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Lipoprotein Transport: Essential for VLDL-transport vesicle fusion with the hepatic cis-Golgi .
Production and Recombinant Variants
Recombinant GOSR1 is produced in multiple expression systems, each tailored for specific research applications:
Key Features:
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Purity: >80% by SDS-PAGE, validated via IMAC or glutathione affinity chromatography .
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Stability: Lyophilized or liquid formulations stored at -80°C to prevent degradation .
Antibody Development
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Recombinant GOSR1 serves as an immunogen for generating monoclonal (e.g., ab53288) and polyclonal antibodies (e.g., PACO65153) .
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Used in Western blot (WB) and immunofluorescence (IF) to study GOSR1 localization in neurodegenerative and cancer models .
Functional Studies
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COPI Displacement: GARP-deficient cells show GOSR1 mislocalization, leading to Golgi fragmentation and impaired retrograde transport .
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Drug Interactions: GOSR1 expression is modulated by caffeine, doxorubicin, and selenium, linking it to chemoresistance pathways .
Disease Associations
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Cancer: Depletion correlates with disrupted Golgi function in hepatocellular carcinoma .
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Neurodegeneration: Protects against hydrogen peroxide-induced cytotoxicity in neuronal cells .
Protein-Protein Interactions
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SNARE Complex: Partners include BET1L (Qb-SNARE), STX5 (Qa-SNARE), and YKT6 (R-SNARE) .
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Regulatory Factors: Binds USO1 (p115) for tethering vesicles to Golgi membranes .
Pathways
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Intra-Golgi Retrograde Transport: Mediated by the GOSR1-STX5-BET1L-YKT6 complex .
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Lipid Homeostasis: GOSR1 depletion alters PI4P and sphingolipid levels, affecting COPI recruitment .
Recent Findings and Future Directions
Product Specs
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Target Background
Golgi SNAP receptor complex member 1 (GOSR1) plays a crucial role in intracellular transport. As a t-SNARE (soluble NSF attachment protein receptor), it facilitates transport from the endoplasmic reticulum (ER) to the Golgi apparatus and intra-Golgi transport. Furthermore, GOSR1 may offer neuroprotection against hydrogen peroxide-induced cytotoxicity by modulating intracellular reactive oxygen species (ROS) levels through p38 MAPK inhibition. Specifically, it participates in ER-to-cis-Golgi docking and fusion and is essential for VLDL (very-low-density lipoprotein) transport vesicle-Golgi fusion, thereby impacting hepatic VLDL delivery to the cis-Golgi.
- Nuclear GOSR1 expression may serve as a prognostic biomarker for colorectal cancer (CRC), potentially identifying aggressive CRC subtypes. PMID: 28638266
- High nuclear GOSR1 expression correlates with poor prognosis in early-stage cervical cancer, suggesting it may be a novel prognostic marker and therapeutic target. PMID: 27611086
- COPI-coated Golgi vesicles inhibit intra-Golgi transport by extracting GOSR1 from Golgi cisternae, thus preventing inter-cisternal connection formation. PMID: 23387339
- GOSR1 might enhance cellular susceptibility to DNA damage-induced apoptosis by inhibiting p53 ubiquitination and degradation. PMID: 22397410
- ORP7 negatively regulates GOSR1 protein stability by sequestering GATE-16, potentially mediating the impact of 25-OH on GOSR1 and Golgi function. PMID: 21669198
- Apolipoprotein E, GRB2, GOSR1, and GBA may contribute to neurodegeneration in stress urinary incontinence. PMID: 20700730
- GOSR1 is part of a SNARE complex (with syntaxin5, Ykt6) involved in ER-to-Golgi and intra-Golgi transport. PMID: 12388752
