Recombinant Human Magnesium transporter protein 1 (MAGT1)
Description
Magnesium Transport
Recombinant MAGT1 facilitates Mg²⁺ uptake in T cells, maintaining cytoplasmic Mg²⁺ levels critical for immune activation. Deficiency impairs CD8⁺ T-cell responses to viral infections (e.g., Epstein-Barr virus) .
B. N-Linked Glycosylation
MAGT1 serves as an accessory subunit of the oligosaccharyltransferase (OST) complex, enabling posttranslational glycosylation of cysteine-proximal sites in glycoproteins . This activity is Mg²⁺-sensitive and partially overlaps with its paralog TUSC3 .
Immune Dysregulation
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MAGT1-deficient mice exhibit accelerated arterial thrombosis, hyperactive platelets, and increased TRPC6-dependent calcium influx .
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In humans, mutations cause XMEN syndrome (X-linked immunodeficiency with Mg²⁺ defect), characterized by chronic EBV infections and lymphoproliferative disorders .
Cancer Associations
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Overexpression in colorectal cancer (CRC) correlates with advanced tumor stages, metastasis, and chemotherapy resistance .
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MAGT1 knockdown reduces CRC cell proliferation and migration in vitro .
Glycosylation Defects
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MAGT1 loss disrupts glycosylation of immune receptors (e.g., CD28) and NKG2D, impairing antiviral responses .
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Mg²⁺ supplementation partially restores glycosylation in MAGT1-deficient cells .
Clinical Relevance
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Gene Therapy: Restoration of MAGT1 in hematopoietic stem cells could correct immune defects in XMEN patients .
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Drug Targets: TRPC6 inhibitors normalize platelet hyperactivity in MAGT1-deficient models .
Experimental Tools
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Antibody Production: MAGT1-specific antibodies (e.g., Proteintech 17430-1-AP) are validated for Western blot, IHC, and IF .
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CRISPR Models: MAGT1-KO cell lines elucidate its roles in Mg²⁺ homeostasis and glycosylation .
Production Challenges
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Solubility: MAGT1’s transmembrane domains require detergent-based extraction for recombinant expression .
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Glycoform Variability: Posttranslational modifications affect molecular weight (38–47 kDa), complicating purification .
Future Directions
Product Specs
Note: We will prioritize shipping the format that we have in stock. However, if you have any specific format requirements, please include them in your order notes. We will prepare according to your request.
Note: All of our proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please contact us in advance as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
- Immunohistochemical evaluation of MAGT1 and AKAP13 expression in clinical hepatocellular carcinoma tissues suggested that both proteins were strongly expressed in tumor tissues. These tissues exhibited significantly higher average immunoreactive scores of Remmele and Stegner (IRS) than non-tumor tissues. PMID: 26617690
- A 0.8 kb intronic duplication in MAGT1 and a single base pair deletion in the last exon of ATRX were identified in a family with five males demonstrating intellectual disability (ID) and unusual skin findings (e.g., generalized pruritus). PMID: 24130152
- A deficiency in magnesium transporter 1 (MAGT1), an Mg(2+)-specific transporter, leads to the absence of a T cell antigen receptor-stimulated Mg(2+)flux and an attenuation of T cell activation. PMID: 21983175
- A novel gene encoding a protein involved in Mg2+-evoked transport, MagT1, was identified. The findings suggest that MagT1 may provide a selective and regulated pathway for Mg2+ transport in epithelial cells. PMID: 15804357
- A study demonstrated that mutations in two OTase-subunit genes, N33/TUSC3 and IAP, result in autosomal-recessive nonsyndromic mental retardation. PMID: 18455129
- MagT1 is universally expressed in all tissues, and its expression level is upregulated in low extracellular Mg(2+). Knockdown of either MagT1 or TUSC3 protein significantly lowers the total and free intracellular Mg(2+) concentrations in cell lines. PMID: 19717468
