Recombinant Human NAD-dependent protein deacetylase sirtuin-1 (SIRT1)
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In Stock 
Description
Production and Purification
Recombinant SIRT1 is typically produced via bacterial expression systems:
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Expression Vector: Cloned into E. coli plasmids for high-yield production .
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Purification: Affinity chromatography using the N-terminal 6xHis tag, achieving >90% purity .
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Activity Validation: Confirmed through deacetylation assays (e.g., p53 or histone H3 substrates) .
Key Functional Roles in Research
Recombinant SIRT1 is widely used to study its roles in:
Apoptosis Regulation
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p53 Deacetylation: Suppresses apoptosis by deacetylating p53 at Lys379, reducing its transcriptional activity .
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Bcl-2/Bax Balance: Enhances Bcl-2 expression while reducing Bax, promoting cell survival .
Metabolic and Stress Adaptation
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PGC-1α/ERR-α Complex: Regulates gluconeogenesis and mitochondrial biogenesis .
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DNA Repair: Facilitates homologous recombination (HR) via interaction with WRN helicase .
Inflammatory and Oxidative Stress
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NF-κB Inhibition: Deacetylates RelA/p65 subunit, dampening inflammatory responses .
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S-Nitrosation Inhibition: Cysteine residues (e.g., C395/C398) are transnitrosated by S-nitrosoglutathione, reversibly inhibiting activity .
Research Applications and Findings
Pharmacological Modulation
Product Specs
Form
Lyophilized powder
Note: We prioritize shipping the format currently in stock. For specific format requirements, please indicate this in your order notes; we will accommodate your request to the best of our ability.
Note: We prioritize shipping the format currently in stock. For specific format requirements, please indicate this in your order notes; we will accommodate your request to the best of our ability.
Lead Time
Delivery times vary depending on the purchase method and location. Please consult your local distributor for precise delivery estimates.
Note: Our proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance; additional charges will apply.
Note: Our proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance; additional charges will apply.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50% and may serve as a guideline for your application.
Shelf Life
Shelf life depends on several factors: storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The specific tag type is determined during production. If you require a particular tag, please specify it; we will prioritize its use during development.
The specific tag type is determined during production. If you require a particular tag, please specify it; we will prioritize its use during development.
Synonyms
75SirT1; hSIR2; hSIRT1; HST2; HST2, S. cerevisiae, homolog of; NAD dependent deacetylase sirtuin 1; NAD dependent protein deacetylase sirtuin 1; NAD-dependent deacetylase sirtuin-1 ; OTTHUMP00000198111; OTTHUMP00000198112; Regulatory protein SIR2 homolog 1; SIR1_HUMAN; SIR2; SIR2 like 1; SIR2 like protein 1; SIR2, S.cerevisiae, homolog-like 1; SIR2-like protein 1; SIR2ALPHA; SIR2L1; Sirt1; SirtT1 75 kDa fragment; Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); Sirtuin 1; Sirtuin type 1
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
2-747
Protein Length
Full Length of Mature Protein
Purity
>85% (SDS-PAGE)
Species
Homo sapiens (Human)
Target Names
Target Protein Sequence
ADEAALALQ PGGSPSAAGA DREAASSPAG EPLRKRPRRD GPGLERSPGE PGGAAPEREV
PAAARGCPGA AAAALWREAE AEAAAAGGEQ EAQATAAAGE GDNGPGLQGP SREPPLADNL
YDEDDDDEGE EEEEAAAAAI GYRDNLLFGD EIITNGFHSC ESDEEDRASH ASSSDWTPRP
RIGPYTFVQQ HLMIGTDPRT ILKDLLPETI PPPELDDMTL WQIVINILSE PPKRKKRKDI
NTIEDAVKLL QECKKIIVLT GAGVSVSCGI PDFRSRDGIY ARLAVDFPDL PDPQAMFDIE
YFRKDPRPFF KFAKEIYPGQ FQPSLCHKFI ALSDKEGKLL RNYTQNIDTL EQVAGIQRII
QCHGSFATAS CLICKYKVDC EAVRGDIFNQ VVPRCPRCPA DEPLAIMKPE IVFFGENLPE
QFHRAMKYDK DEVDLLIVIG SSLKVRPVAL IPSSIPHEVP QILINREPLP HLHFDVELLG
DCDVIINELC HRLGGEYAKL CCNPVKLSEI TEKPPRTQKE LAYLSELPPT PLHVSEDSSS
PERTSPPDSS VIVTLLDQAA KSNDDLDVSE SKGCMEEKPQ EVQTSRNVES IAEQMENPDL
KNVGSSTGEK NERTSVAGTV RKCWPNRVAK EQISRRLDGN QYLFLPPNRY IFHGAEVYSD
SEDDVLSSSS CGSNSDSGTC QSPSLEEPME DESEIEEFYN GLEDEPDVPE RAGGAGFGTD
GDDQEAINEA ISVKQEVTDM NYPSNKS
Uniprot No.
Target Background
Function
Recombinant Human NAD-dependent protein deacetylase sirtuin-1 (SIRT1) is a highly versatile enzyme with a wide range of cellular functions. It acts as a NAD+/NADH sensor, linking transcriptional regulation to cellular energetics. SIRT1 deacetylates histones and various transcription factors and coregulators, influencing gene expression. Its roles include modulation of cell cycle, DNA damage response, metabolism, apoptosis, and autophagy. SIRT1 is a key component of the eNoSC (energy-dependent nucleolar silencing) complex, regulating rDNA silencing in response to energy status. It interacts with numerous proteins, affecting processes such as skeletal muscle differentiation, genomic integrity, and responses to oxidative and metabolic stress. SIRT1's deacetylation activity regulates the function of key proteins involved in diverse pathways, including p53, FOXO proteins, NF-κB, and many others. Its influence extends to lipid metabolism, adipogenesis, insulin secretion, DNA repair, autophagy, and circadian rhythm regulation. SIRT1's functional versatility underscores its importance in maintaining cellular homeostasis and its implications in various physiological and pathological conditions.
Gene References Into Functions
- Resveratrol induces chondrosarcoma cell apoptosis via SIRT1-activated NF-κB (p65 subunit) deacetylation, exhibiting in vivo anti-chondrosarcoma activity. PMID: 28600541
- I157172 upregulates SIRT1 and downregulates acetyl-STAT3. PMID: 30365149
- SIRT1-mediated H3K9 deacetylation maintains gene repression but is not essential for direct ZEB2 repression; SIRT1 activity maintains the stability of ZEB2-induced RAB25 repression. PMID: 30445998
- Long non-coding RNA MALAT1 represses SIRT1 expression by targeting Foxo1. PMID: 29928873
- SIRT1 plays a protective role in regulating ADSCs aging and H2O2-induced apoptosis. PMID: 29803744
- Plasma SIRT1 levels inversely correlate with adiposity and atherogenicity indices in metabolic syndrome patients. PMID: 29779969
- Increased SIRT1 activity protects against diabetes-induced podocyte injury, mitigating diabetic kidney disease progression. PMID: 29477240
- The Sirt1 carboxyl-domain is an ATP-repressible domain transferable to other proteins. PMID: 28504272
- Adipose tissue, but not muscle, sirtuin 1 is related to insulin sensitivity, independent of BMI but dependent on adipose tissue SLC2A4. PMID: 29417372
- SIRT1 acts as an oncogenic factor in breast cancer, potentially by inhibiting p53 and activating POLD1. PMID: 29807012
- SIRT1 is upregulated in paclitaxel-resistant cervical cancer, and its knockdown inhibits cell proliferation, promotes cell cycle arrest and apoptosis, and decreases MDR protein expression. PMID: 29808798
- High SIRT1 expression correlates with vascular invasion and worse prognosis in colorectal cancer. PMID: 30082156
- SIRT1 polymorphisms and expression are associated with alcoholic fatty liver disease (AFLD), with relationships between four SNPs and BMI in AFLD patients, but no SNP relation to expression. PMID: 29189974
- SIRT1 plays a variable role in mesenchymal stem cell maintenance and differentiation. PMID: 29715067
- SIRT1 promotes chondrosarcoma metastasis by inducing epithelial-mesenchymal transition. PMID: 28112277
- TM4SF1 regulation in bladder cancer is influenced by a PPARγ-SIRT1 feedback loop. PMID: 29175458
- SIRT1 regulates CLDN5 transcription, possibly by modifying KLF4 activity in ovarian cancer cells. PMID: 28888043
- Seminal SIRT1 expression is implicated in male infertility. PMID: 29359516
- SIRT1 promotes tumor cell transformation by inducing epithelial-mesenchymal transition. PMID: 29656187
- The Sirt1-Smad2 interaction is relevant for regulating TGFβ-dependent gene transcription. PMID: 29187201
- miR-29a targets SIRT1 mRNA 3'-UTR, regulating its protein expression; increased SIRT1 expression rescues miR-29a overexpression effects in HCC cells. PMID: 29630527
- High Sirt1 expression is associated with gastric cancer. PMID: 29693338
- Sirt1 protects against oxidative stress-induced apoptosis in psoriatic patient fibroblasts. PMID: 29799444
- Statin use in young PMI patients is associated with higher SIRT1 and lower eNOS protein levels compared to controls. PMID: 29664427
- SIRT1 is a crucial regulator of hepatocellular function in human liver transplants under ischemia/reperfusion stress. PMID: 28719070
- SIRT1 predicts poor prognosis in esophageal squamous cell carcinoma, potentially linked to EGFR overexpression. PMID: 29625788
- A novel mechanism for SIRT1 activation under stress conditions suggests a therapeutic target for age-related diseases. PMID: 29133780
- Sirt1 and senescence regulate beige adipocyte differentiation during aging. PMID: 29678576
- Baicalin activates SIRT1/AMPK and mTOR signaling in A549 and H1299 cells; SIRT1 and AMPK silencing reduces baicalin effects on cell proliferation and migration. PMID: 29632297
- SIRT1 and SIRT6 dampen inflammation and oxidative stress in the cardiovascular system. PMID: 28661724
- SIRT1 is a direct target of miR-212; its expression inversely correlates with miR-212 in thyroid cancer; SIRT1 overexpression rescues miR-212 mimic-induced suppression in TPC-1 cells. PMID: 29207181
- SIRT1 polymorphisms are associated with antisocial personality in adolescents. PMID: 28439078
- Dexamethasone downregulates SIRT1 and IL6 but upregulates EDN1 in gingival-derived aging stem cells. PMID: 29302812
- miR-200a and SIRT1 may induce apoptosis in dopaminergic neurons. PMID: 29936262
- A rhuschalcone I analogue (9) shows sirt1 inhibitory activity (IC50 = 40.8 µM). PMID: 29443909
- SIRT1 contributes to salidroside's neuroprotection against MPP(+)-induced apoptosis and oxidative stress by suppressing MAPK pathways. PMID: 28851138
- Decreased SIRT1 expression and SUMOylation are associated with bronchopulmonary dysplasia. PMID: 29115559
- SIRT1 negatively regulates poly(A)RNA transport by deacetylating PABP1. PMID: 28756945
- L-Carnitine alleviates perfluorooctanesulfonate-induced renal fibrosis via a Sirt1- and PPARγ-dependent mechanism. PMID: 28973641
- High SIRT1 expression is associated with hepatocellular carcinoma. PMID: 28677784
- No association exists between SIRT1 polymorphisms and diabetic foot severity. PMID: 29995800
- Physical activity level correlates with peripheral blood mononuclear cell SIRT1 and FOXO1 mRNA expression in COPD patients. PMID: 29138552
- Defective sirtuin-1 increases IL-4 expression via GATA-3 acetylation in severe asthma. PMID: 26627546
- SIRT1 gene polymorphisms affect coronary artery disease pathogenesis. PMID: 29885463
- miR-146 exerts protective functions by upregulating Sirt1, blocking NF-κB and Notch pathways. PMID: 29229881
- SIRT1 deficiency in bladder cancer cells suppresses cell viability by activating antioxidant response and inducing cell cycle arrest, possibly via FOXO3a pathways. PMID: 29147649
- miR-200a suppresses SIRT1 upregulation in renal cell carcinoma (RCC), highlighting its tumor-suppressive role. PMID: 28717923
- RSV or SIRT1 overexpression inhibits NMDA-induced excitotoxicity. PMID: 29081884
- FOXO1 inhibits gastric cancer growth and angiogenesis under hypoxia via the HIF-1α-VEGF pathway, possibly involving SIRT1. PMID: 25761483
- Resveratrol restores activity toward certain “loose-binding” SIRT1 substrates, informing the design of substrate-specific SIRT1 modulators. PMID: 27901083
Database Links
Protein Families
Sirtuin family, Class I subfamily
Subcellular Location
Nucleus, PML body. Cytoplasm. Nucleus.; [SirtT1 75 kDa fragment]: Cytoplasm. Mitochondrion.
Tissue Specificity
Widely expressed.
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