Recombinant Human Probable G-protein coupled receptor 171 (GPR171)
Description
Production and Quality Control
Recombinant GPR171 is generated using heterologous expression systems to ensure high purity and functionality:
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Expression Systems: Common platforms include E. coli, wheat germ, and mammalian cells. For example, Creative BioMart’s GPR171-5210H variant is expressed in wheat germ without affinity tags .
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Storage: Requires storage at -80°C in 25 mM Tris-HCl buffer (pH 8.0) with 2% glycerol to prevent aggregation .
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Validation: Functional assays (e.g., GTPγS binding, cAMP modulation) confirm receptor activity .
Research Applications
Recombinant GPR171 serves as a critical tool in both basic and translational research:
Key Findings from Preclinical Studies
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Metabolic Regulation:
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Immune Modulation:
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Cancer Progression:
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Pain Pathways:
Clinical and Therapeutic Implications
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Target Validation: GPR171’s dual role in metabolism and immunity positions it as a multipurpose target for obesity, cancer, and autoimmune diseases.
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Drug Development: Synthetic ligands (e.g., MS15203) show promise in preclinical pain models but require further optimization for human trials .
Challenges and Future Directions
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific requirements for the format, please indicate them in your order. We will prepare the product according to your specifications.
Note: All proteins are shipped with standard blue ice packs. If you require dry ice shipping, please contact us in advance. Additional fees may apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Target Background
- High GPR171 expression enhances proliferation and metastasis of lung cancer. PMID: 26760963
