Recombinant Human Protein FAM26E (FAM26E)
Description
Biophysical and Functional Properties
FAM26E functions as a voltage- and calcium-regulated ion channel with broad permeability to cations and signaling molecules like ATP . Key findings include:
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Voltage gating: Activation at depolarized membrane potentials, modulated by extracellular Ca²⁺ ([Ca²⁺]₀) .
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Ion selectivity: Poor discrimination among cations (e.g., Ca²⁺, Na⁺) and anions, enabling Ca²⁺ influx and ATP release .
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Regulatory mechanism: Extracellular Ca²⁺ acts as an allosteric modulator, reducing channel open probability at physiological concentrations (~1.5 mM) .
Table 2: Functional Comparisons with CALHM1
Research Applications and Recombinant Variants
Recombinant FAM26E is widely used for mechanistic studies and antibody validation. Key variants include:
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Full-length constructs: Expressed in mammalian systems (e.g., HEK293) with tags (His, Fc-Avi) for purification .
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Control fragments: Truncated proteins (e.g., aa 218–309) for blocking experiments in immunohistochemistry (IHC) and Western blotting (WB) .
Table 3: Available Recombinant FAM26E Products
| Expression System | Tag | Purity | Applications | Source |
|---|---|---|---|---|
| Mammalian Cells (HEK293) | His, Fc-Avi | >80% | Structural studies, assays | Creative BioMart |
| E. coli | His | >90% | Antibody validation | Thermo Fisher |
Research Findings and Pathways
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Cation channel activity: FAM26E interacts with TRPM1, ASIC3, and CALHM2/3 in cation transport pathways .
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Pathway associations: Implicated in calcium signaling and neuronal excitability, though specific pathways remain under investigation .
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Disease links: Polymorphisms in CALHM1 (a homolog) correlate with Alzheimer’s disease onset, suggesting potential neurodegenerative roles for CALHM5 .
Key Reagents and Tools
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Antibodies: Validated for WB, IHC, and flow cytometry (e.g., ABIN651998, ABIN1812617) .
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Interacting proteins: Co-immunoprecipitation studies suggest associations with dynein and exocyst complex components .
Challenges and Future Directions
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them during order placement, and we will accommodate your request.
Note: All our proteins are shipped with standard blue ice packs. If dry ice shipping is preferred, please notify us in advance. Additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
