Recombinant Human Protein phosphatase 1 regulatory subunit 42 (PPP1R42)
Description
Introduction to Recombinant Human Protein Phosphatase 1 Regulatory Subunit 42 (PPP1R42)
Recombinant Human Protein Phosphatase 1 Regulatory Subunit 42 (PPP1R42) is a protein that plays a crucial role in regulating the activity of Protein Phosphatase 1 (PP1), a key enzyme involved in various cellular processes. PPP1R42 is particularly noted for its involvement in centrosome dynamics and spermatogenesis. This article provides an overview of PPP1R42's function, its role in cellular processes, and recent research findings.
Function and Localization of PPP1R42
PPP1R42 is localized to the centrosome and basal body of primary cilia in cells such as the retinal pigmented epithelial cell line ARPE-19 . It interacts with PP1 to regulate centrosome separation, which is essential for maintaining proper cell division and cilia formation . PPP1R42 acts as a positive regulator of PP1 activity, and its depletion can lead to increased centrosome separation due to the activation of NEK2 kinase .
| Localization | Function |
|---|---|
| Centrosome and Basal Body | Regulates Centrosome Separation |
| Spermatids | Involved in Spermatogenesis |
Role in Cellular Processes
PPP1R42 is involved in several cellular processes, including cell cycle regulation and apoptosis. Depletion of PPP1R42 leads to an increase in cells with multiple centrosomes, which can disrupt normal cell cycle progression and lead to increased cell death . PPP1R42's interaction with PP1 is crucial for maintaining the balance of phosphorylation and dephosphorylation events necessary for proper cell function.
Cell Cycle Impact
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G2 Phase Increase: PPP1R42 knockdown results in a significant increase in cells in the G2 phase, indicating a disruption in cell cycle progression .
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Apoptosis: There is a notable increase in dead cells following PPP1R42 depletion, suggesting a role in cell survival .
Research Findings
Recent studies have highlighted PPP1R42's role in centrosome dynamics and its interaction with PP1. The protein's localization to the centrosome and its involvement in spermatogenesis underscore its importance in reproductive biology . Additionally, PPP1R42's ability to regulate PP1 activity suggests it plays a broader role in cellular signaling pathways.
| Study | Key Findings |
|---|---|
| Wang et al., 2010 | PPP1R42 interacts with PP1 at the centrosome of spermatids . |
| Wang et al., 2013 | PPP1R42 depletion increases centrosome number and affects cell cycle progression . |
References Wang et al. (2013). PPP1R42, a PP1 binding protein, regulates centrosome dynamics in ciliated ARPE-19 cells. NCBI Gene: PPP1R42 protein phosphatase 1 regulatory subunit 42. NCBI Gene: PPP1R42 protein phosphatase 1 regulatory subunit 42. GeneCards: PPP1R42 Gene - Protein Phosphatase 1 Regulatory Subunit 42.
Product Specs
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Target Background
- PPP1R42's role in regulating PP1 function during centrosome separation. PMID: 23718219
