Recombinant Human Protein SCAI (SCAI)
Description
Introduction
SCAI, or Suppressor of Cancer Cell Invasion, is a highly conserved protein among vertebrates that has been implicated in transcriptional regulation . It lacks annotated domains and shares little sequence homology with other proteins . Research has shown that SCAI plays a significant role in DNA double-strand break (DSB) repair, response to UV-induced replicative stress, and error-free repair of DNA interstrand crosslinks (ICLs) .
SCAI's Role in DNA Repair
SCAI promotes DNA double-strand break repair through both non-homologous end-joining (NHEJ) and homologous recombination (HR) pathways, which are essential for maintaining genome integrity after DNA damage .
-
DSB Repair: SCAI is enriched at DSB sites through 53BP1-dependent recruitment to DSB-surrounding chromatin and 53BP1-independent accumulation at resected DSBs . Cells lacking SCAI show reduced DSB repair capacity, increased sensitivity to DSB-inflicting agents, and genome instability . SCAI facilitates ATM kinase signaling at DSBs in repressive chromatin environments, acting as a mediator of 53BP1-dependent repair of heterochromatin-associated DSBs .
-
UV-Induced Replicative Stress Response: SCAI functions as a regulator of the replicative stress response caused by UV exposure . Cells with SCAI knocked down or knocked out exhibit elevated RPA-ssDNA post-UV, suggesting its involvement in the response to replicative stress . SCAI colocalizes with stalled replication forks, and its depletion promotes nascent DNA degradation. SCAI inhibits EXO1 activity on a single-stranded DNA gap in vitro, indicating its role in preventing excessive DNA resection .
-
ICL Repair: SCAI plays a critical role in ICL repair via the FA pathway . Mass spectrometry analysis has identified interactions between SCAI and Fanconi Anemia (FA) proteins, as well as transcription and chromatin remodeling factors. SCAI interacts with all five subunits of the polymerase ζ (Polζ) complex (REV1, REV3, REV7, POLD2, and POLD3) .
SCAI and Cancer Cell Invasion
SCAI was initially identified as a suppressor of cancer cell invasion . High SCAI expression is associated with papillary thyroid cancer cells .
SCAI-Interacting Proteins
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order remarks for customized preparation.
Note: All proteins are shipped with standard blue ice packs unless dry ice is specifically requested in advance. Additional fees apply for dry ice shipping.
Note: While the tag type is determined during production, we prioritize specific tag requests if communicated in advance.
Target Background
- High SCAI expression correlates with gastric, prostate, and colorectal cancers. PMID: 28815470
- Whole-genome and Sanger sequencing confirmed SMAD4 gene integration within the last intron of the SCAI gene in five cases. PMID: 28867604
- SCAI inhibits RIF1, enabling BRCA1-mediated DNA repair, potentially including alternative non-homologous end joining (alt-NHEJ), resection-dependent NHEJ in G1, and homologous recombination repair (HDR) in S/G2 phases. PMID: 28700933
- miR-625-3p exhibits oncogenic effects through regulation of the SCAI/E-cadherin/MMP-9 pathways. PMID: 26314959
- miR-1228 promotes breast cancer proliferation, invasion, and migration through MOAP1 and SCAI. PMID: 26261546
- SCAI downregulation enhances glioma cell invasion and cancer stem cell-like phenotypes by activating Wnt/β-catenin signaling. PMID: 24785374
- SCAI is a specific binding partner for KMD3B. PMID: 23593242
- SCAI is a transcriptional co-factor regulating epithelial-to-mesenchymal transition and renal fibrosis. PMID: 23178076
- Clinical trial data on gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
- SCAI suppression strongly upregulates β1-integrin expression. PMID: 19350017
