Recombinant Human Receptor expression-enhancing protein 3 (REEP3)
Description
General Information
Receptor expression-enhancing protein 3 (REEP3) is a protein that, in humans, is encoded by the REEP3 gene . REEP3 is also associated with autism susceptibility . Additionally, REEP3 and REEP4 determine the tubular morphology of the endoplasmic reticulum . Reverse transcriptase (RT-PCR) has demonstrated that BC068557 and BC018658 mRNAs correspond to the same REEP3 gene .
Function and Characterization
REEPs are membrane-shaping adapter proteins that modulate specific G protein-coupled receptor trafficking by affecting ER cargo capacity . REEP3 and REEP4 function redundantly to remove the endoplasmic reticulum (ER) from mitotic chromosomes .
Role in Mitotic ER Shaping
REEP3/4 mediate high curvature of the ER specifically during mitosis . REEP4 protein levels were increased by approximately 50% in a mitotically enriched cell population, whereas REEP3 protein levels remained nearly unchanged . REEP3/4 contain residues in their C-terminal regions that promote mitosis-specific ER shaping and may be targets of regulatory inputs .
After REEP3/4 RNAi, large sheets appeared, which were fenestrated to a similar degree as the remaining sheet-like regions in control cells . ER profiles were distributed throughout the entire cell and extended into the spindle area .
Product Specs
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Target Background
- REEP3 and REEP4 redundantly clear the ER from metaphase chromatin, ensuring proper mitotic progression and nuclear envelope architecture. PMID: 23911198
- Clinical trial data on gene-disease associations and gene-environment interactions (HuGE Navigator). PMID: 20379614
- REEP3, a human homolog of yeast Yop1p, may regulate ER-Golgi vesicle trafficking. It's also a positional candidate gene for autism. PMID: 17290275
- Observational study of gene-disease associations (HuGE Navigator). PMID: 16385451
- Observational study, meta-analysis, and genome-wide association study of gene-disease associations (HuGE Navigator). PMID: 18940312
Q&A
Here’s a structured FAQ collection for researchers studying Recombinant Human REEP3, incorporating experimental design considerations, methodological insights, and data-driven analysis:
Advanced Research Questions
How to resolve contradictions in REEP3’s role in ER morphology during mitosis?
How does REEP3 influence immune infiltration in pancreatic cancer?
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Correlation: REEP3 upregulation correlates with CD8+ T cells (r = 0.484, p = 2.1×10⁻¹¹) and dendritic cells (r = 0.409, p = 2.84×10⁻⁸) .
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Functional assay: Knockdown REEP3 in Panc-1 cells and profile cytokines via Luminex to identify immune recruitment pathways .
What strategies optimize recombinant REEP3 expression in HEK-293 systems?
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Temperature modulation: Shift cultures to 33°C post-transfection to arrest cells in G₀/G₁, boosting yield by 1.5× .
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Transfection comparison:
Reagent DNA (µg) Efficiency Lipofectamine 2000 0.5 High Calcium phosphate 5.0 Comparable
Data Contradiction Analysis
Why do REEP3 knockout studies show divergent phenotypes in ER-chromatin clearance?
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Key variables:
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Validation: Perform co-immunoprecipitation (Co-IP) with REEP3/4 and quantify ER-chromatin overlap via 3D confocal reconstruction .
