Recombinant Human Taste receptor type 2 member 43 (TAS2R43)
Description
Production Methods
Recombinant TAS2R43 is synthesized using advanced platforms such as Magic™ Membrane Protein Production (Creative Biolabs) :
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Expression Systems: Mammalian (e.g., HEK-293) and insect cell lines for proper post-translational modifications .
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Purification: Affinity chromatography with tags (e.g., GST) and stabilization using lipid nanodiscs .
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Validation: Functional assays (calcium imaging) confirm receptor activity .
Challenges:
Ligand Specificity and Signaling
Recombinant TAS2R43 responds to diverse agonists and antagonists:
Genetic Polymorphisms
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W35S/H212R Variants: Reduce receptor activity by >90%, correlating with lower caffeine bitterness perception and altered coffee preference .
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Population Diversity: Over 166 predicted functional variants exist, though most are rare (frequency <0.01) .
Extraoral Functions
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Gut Physiology: Mediates caffeine-induced gastric acid secretion via parietal cells .
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Immune Modulation: Potential role in detecting bacterial quorum-sensing molecules .
Drug Development
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Bitter Masking Agents: HED and structural analogs inhibit TAS2R43 to mitigate drug aftertastes .
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Toxicity Screening: Detects aristolochic acid exposure, a carcinogenic contaminant .
Food Science
Disease Research
Product Specs
Note: We will prioritize shipping the format currently in stock. If you have specific format requirements, please specify them during order placement.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type is determined during the production process. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
Gustducin-coupled receptor involved in the detection of bitter compounds within the oral cavity and gastrointestinal tract. It signals through PLCB2 and the calcium-regulated cation channel TRPM5. Activated by the artificial sweeteners saccharin and acesulfame K. In airway epithelial cells, bitter compound binding increases intracellular calcium ion concentration and stimulates ciliary beat frequency. It may function as a chemosensory receptor in airway epithelial cells, facilitating the detection and removal of potentially harmful agents from the airways.
- Individuals with at least one sensitive TAS2R38 allele (AP or PP genotype) exhibited a higher likelihood of rejecting liquid medications compared to those with the non-taster allele (AA genotype). PMID: 26391354
- This study highlights the distinct response profiles of feline bitter receptors Tas2r38 and Tas2r43 compared to their human orthologs. PMID: 26037485
- The influence of the TAS2R43 gene on coffee preference is mediated by caffeine, particularly the H212R variant. PMID: 24647340
- Research indicates that bitter compounds elicit specific physiological effects in cells expressing type 2 taste receptors (T2Rs). PMID: 22964302
- The TAS2R43 mutational polymorphism significantly impacts susceptibility to BEN, suggesting a potentially broad role for toxin responses in individual health risks. PMID: 23050764
- Our findings demonstrate a significant increase in the expression rate of several T2R taste receptor genes in patients with phantogeusia. PMID: 22397221
- hTAS2R43 and hTAS2R44 function as cognate bitter taste receptors and do not contribute to the sweet taste of saccharin and acesulfame K. PMID: 15537898
- The hT2R43 gene allele enhances sensitivity to the bitterness of saccharin; similarly, an allele of a closely related gene (hT2R44) also increases saccharin bitterness sensitivity. PMID: 17702579
