Recombinant Human C-X-C motif chemokine 17 (CXCL17)

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Cat. No.
BT1186467
Source
Yeast
Synonyms
CXCL17; VCC1; UNQ473/PRO842C-X-C motif chemokine 17; 6-Cys CXCL17; Dendritic cell and monocyte chemokine-like protein; DMC; VEGF coregulated chemokine 1) [Cleaved into: 4-Cys CXCL17]
Purity
>85% (SDS-PAGE)
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Cat. No.
BT1186467
Source
E.coli
Synonyms
CXCL17; VCC1; UNQ473/PRO842C-X-C motif chemokine 17; 6-Cys CXCL17; Dendritic cell and monocyte chemokine-like protein; DMC; VEGF coregulated chemokine 1) [Cleaved into: 4-Cys CXCL17]
Purity
>85% (SDS-PAGE)
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Cat. No.
BT1186467
Source
E.coli
Synonyms
CXCL17; VCC1; UNQ473/PRO842C-X-C motif chemokine 17; 6-Cys CXCL17; Dendritic cell and monocyte chemokine-like protein; DMC; VEGF coregulated chemokine 1) [Cleaved into: 4-Cys CXCL17]
Purity
>85% (SDS-PAGE)
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Cat. No.
BT1186467
Source
Baculovirus
Synonyms
CXCL17; VCC1; UNQ473/PRO842C-X-C motif chemokine 17; 6-Cys CXCL17; Dendritic cell and monocyte chemokine-like protein; DMC; VEGF coregulated chemokine 1) [Cleaved into: 4-Cys CXCL17]
Purity
>85% (SDS-PAGE)
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Cat. No.
BT1186467
Source
Mammalian cell
Synonyms
CXCL17; VCC1; UNQ473/PRO842C-X-C motif chemokine 17; 6-Cys CXCL17; Dendritic cell and monocyte chemokine-like protein; DMC; VEGF coregulated chemokine 1) [Cleaved into: 4-Cys CXCL17]
Purity
>85% (SDS-PAGE)
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Description

Overview of Recombinant Human CXCL17

Recombinant Human CXCL17 is a laboratory-engineered version of the naturally occurring chemokine CXCL17, which belongs to the CXC chemokine family. First identified in 2006, CXCL17 is also termed VEGF coregulated chemokine 1 (VCC-1) or dendritic cell- and monocyte-attracting chemokine-like protein (DMC) . The recombinant form is produced in Escherichia coli (E. coli) and retains biological activities such as chemotaxis for monocytes, macrophages, and dendritic cells, along with roles in tumor angiogenesis .

Production and Purification

Recombinant CXCL17 is synthesized in E. coli using codon-optimized DNA sequences. Post-expression, it undergoes chromatographic purification (e.g., affinity chromatography for His-tagged variants) and lyophilization. Key steps include:

  • Expression: Induced in E. coli BL21(DE3) strains .

  • Purification: Nickel-affinity chromatography followed by buffer exchange .

  • Quality Control: Validated via SDS-PAGE (>85% purity) and endotoxin testing (<0.1 EU/μg) .

Chemoattractant Activity

CXCL17 recruits monocytes, immature dendritic cells, and macrophages via GPR35/CXCR8, activating ERK1/2 and p38 signaling pathways . Its chemotactic potency is concentration-dependent, with an ED50 <5 μg/mL in murine endothelial cell assays .

Angiogenic and Anti-Inflammatory Roles

  • Induces VEGF and FGF production in endothelial cells, promoting tumor vascularization .

  • Exhibits anti-inflammatory effects in gastric mucosa by suppressing LPS-activated macrophages .

Oncogenic Implications

  • Pro-Tumor Effects: Overexpression in A549 lung cancer cells enhances migration (>60% wound closure at 24 hours) and invasion . Correlated with poor prognosis in breast (60% CXCL17+ cells) and colorectal cancers (50% CXCL17+ cells) .

  • Therapeutic Target: Linked to drug resistance in colorectal cancer and Akt pathway activation in cervical cancer .

In Vitro Studies

Study FocusKey FindingSource
Monocyte ChemotaxisERK1/2 and p38 pathways mediate CXCL17-induced migration
Tumor AngiogenesisUpregulates VEGF in endothelial cells; ED50 = 5 μg/mL
Lung Cancer MetastasisCXCL17 overexpression increases A549 cell migration by 25–30%

Clinical and Translational Relevance

CXCL17 exhibits dual roles in disease:

  • Protective: Limits viral infections and autoimmune encephalomyelitis .

  • Pathogenic: Drives progression in pulmonary fibrosis, asthma, and hepatocellular carcinoma .
    Current research explores CXCL17 as a biomarker for cancer prognosis and a target for anti-angiogenic therapies .

Product Specs

Form
Lyophilized powder
Note: We will prioritize shipping the format currently in stock. If you have specific format requirements, please specify them during order placement. We will fulfill your request to the best of our ability.
Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs unless otherwise requested. Dry ice shipping requires prior communication and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Before opening, briefly centrifuge the vial to collect the contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a reference for customers.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and the protein's inherent stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during the manufacturing process.
Tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
CXCL17; VCC1; UNQ473/PRO842C-X-C motif chemokine 17; 6-Cys CXCL17; Dendritic cell and monocyte chemokine-like protein; DMC; VEGF coregulated chemokine 1) [Cleaved into: 4-Cys CXCL17]
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
22-119
Protein Length
Full Length of Mature Protein
Purity
>85% (SDS-PAGE)
Species
Homo sapiens (Human)
Target Names
CXCL17
Target Protein Sequence
SSLNPGVAR GHRDRGQASR RWLQEGGQEC ECKDWFLRAP RRKFMTVSGL PKKQCPCDHF KGNVKKTRHQ RHHRKPNKHS RACQQFLKQC QLRSFALPL
Uniprot No.
Q6UXB2

Target Background

Function
Chemokine acting as a chemoattractant for monocytes, macrophages, and dendritic cells. It plays a role in angiogenesis and potentially tumor development. It exhibits anti-inflammatory effects in the stomach and may contribute to innate infection defense. Activation of the C-X-C chemokine receptor GPR35 induces a rapid, transient increase in intracellular calcium levels. It demonstrates significantly higher chemoattractant potency on monocytes and macrophages compared to 6-Cys CXCL17.
Gene References Into Functions
  1. High lymph node CXCL17 mRNA levels are associated with colon cancer. PMID: 30198422
  2. High CXCL17 expression correlates with reduced overall survival in breast cancer patients. CXCL17 interacts with CXCR8 in breast cancer cells. PMID: 28943434
  3. CXCL17 attenuates imiquimod-induced psoriasis-like skin inflammation by recruiting myeloid-derived suppressor cells and regulatory T cells, potentially regulating excessive inflammation in psoriasis. PMID: 28389593
  4. This study identifies GPR35 as the CXCL17 receptor. PMID: 25411203
  5. CXCL17 production correlates with adverse immune infiltration and may be a significant target for anti-HCC therapies. PMID: 25303284
  6. CXCL17 is identified as an antimicrobial mucosal chemokine potentially involved in interstitial lung disease pathogenesis. PMID: 22611239
  7. Evidence suggests VCC-1's involvement in cisplatin-induced apoptosis of HepG2 cells, indicating its potential as a chemotherapy target. PMID: 22425983
  8. A 35kDa Fc-fusion protein with a modified human immunoglobulin IgG1 Fc domain inhibits CC chemokine-induced calcium flux, chemotaxis, and beta-arrestin recruitment in primary macrophages and transfected cells. PMID: 21586597
  9. Immune surveillance during early intraepithelial stages of human pancreatic carcinogenesis involves CXCL17 and ICAM2 expression. PMID: 20955708
  10. DMC induces monocyte and immature dendritic cell migration. Constitutive DMC expression in the lung suggests a role in recruiting these cells from blood to lung parenchyma. PMID: 16455961
  11. VCC1 plays a role in angiogenesis and potentially tumor development in certain tissues. PMID: 16989774
  12. VCC-1 plays a significant role in hepatocellular carcinoma cell invasion and tumor growth. PMID: 19657564
Database Links

HGNC: 19232

OMIM: 611387

KEGG: hsa:284340

STRING: 9606.ENSP00000472467

UniGene: Hs.445586

Protein Families
Intercrine alpha (chemokine CxC) family
Subcellular Location
Secreted.
Tissue Specificity
Detected in trachea, stomach, lung and skeletal muscle. Detected in intestine and in normal and asthmatic lung (at protein level). Breast tumors showed 3- to 24-fold up-regulation.

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