Recombinant Huperzia lucidula Photosystem II CP47 chlorophyll apoprotein (psbB)
Description
Production and Purification
The protein is typically expressed in E. coli systems with N-terminal His-tags for affinity purification . Commercial vendors optimize yields using Tris/PBS-based buffers with glycerol or trehalose for stability .
Production Metrics:
| Parameter | Specification |
|---|---|
| Expression Host | E. coli |
| Purity | >85–90% (SDS-PAGE) |
| Storage | Lyophilized at -20°C/-80°C |
| Reconstitution | 0.1–1.0 mg/mL in sterile water + glycerol |
Comparative Genomic Analysis
The psbB gene in H. lucidula exhibits evolutionary conservation with other lycophytes. A comparative analysis of chloroplast genomes highlights distinct features :
Chloroplast Genome Features:
| Feature | H. lucidula | H. serrata |
|---|---|---|
| Total Size (bp) | 154,373 | 154,176 |
| LSC Region (bp) | 104,088 | 104,080 |
| SSC Region (bp) | 19,657 | 19,658 |
| GC Content (%) | 36.3 | 36.3 |
| Protein-Coding Genes | 86 | 86 |
Notably, H. lucidula lacks the trnI-GAU and ycf66 intron found in H. serrata, suggesting lineage-specific adaptations .
Applications in Photosynthesis Research
-
Mechanistic Studies: Used to probe chlorophyll-protein interactions and PSII repair cycles under photodamage .
-
Evolutionary Biology: Serves as a molecular marker for studying chloroplast genome rearrangements in lycophytes .
-
Biotechnology: Templates for engineering synthetic light-harvesting complexes .
Limitations and Future Directions
While the recombinant protein replicates structural features, it does not natively bind chlorophylls or xanthophylls, limiting functional studies in vitro . Future work could focus on co-expressing chaperones or utilizing eukaryotic expression systems to improve pigment incorporation .
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them when placing the order. We will prepare according to your request.
Note: All our proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please inform us in advance. Additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
