Recombinant Huperzia lucidula Photosystem II reaction center protein Z (psbZ)
Description
Introduction to Recombinant Huperzia lucidula Photosystem II Reaction Center Protein Z (psbZ)
The recombinant Huperzia lucidula Photosystem II reaction center protein Z (psbZ) is a full-length, His-tagged protein derived from the club moss Huperzia lucidula. Expressed in E. coli, this protein corresponds to the UniProt accession Q5SD09 and is part of the Photosystem II (PSII) complex, critical for light-driven water oxidation in photosynthesis .
Expression and Purification
| Parameter | Specification |
|---|---|
| Source | Escherichia coli |
| Tag | N-terminal His-tag |
| Purity | >90% (SDS-PAGE validated) |
| Form | Lyophilized powder |
| Storage Buffer | Tris/PBS-based buffer with 6% trehalose, pH 8.0 |
| Reconstitution | 0.1–1.0 mg/mL in deionized sterile water; add 5–50% glycerol for long-term storage |
Table 1: Key Specifications of Recombinant psbZ
Functional Role in Photosynthesis
psbZ is a low-molecular-weight subunit of the PSII core complex, contributing to the stability and assembly of light-harvesting and reaction center components. In Huperzia lucidula, the psbZ gene is part of the plastid genome, which exhibits minimal structural rearrangements compared to ancestral land plants . The plastome retains collinearity with nonvascular plants, suggesting evolutionary conservation of psbZ’s genomic context .
Comparative Genomic Context
In lycophytes like H. lucidula, plastome evolution involves minor IR expansions capturing genes such as ndhF and rps12, but psbZ remains in its conserved position within the large single-copy (LSC) region . This contrasts with Selaginella and Isoetes, where plastome rearrangements are more pronounced .
Product Specs
Please note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them during order placement, and we will fulfill your request.
Note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance as additional charges will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. For lyophilized form, the shelf life is 12 months at -20°C/-80°C.
