Recombinant Ictalurid herpesvirus 1 Putative zinc-binding protein ORF9 (ORF9)

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Cat. No.
BT43917
Source
Yeast
Synonyms
ORF9; Putative zinc-binding protein ORF9
Purity
>85% (SDS-PAGE)
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Cat. No.
BT43917
Source
E.coli
Synonyms
ORF9; Putative zinc-binding protein ORF9
Purity
>85% (SDS-PAGE)
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Cat. No.
BT43917
Source
E.coli
Synonyms
ORF9; Putative zinc-binding protein ORF9
Purity
>85% (SDS-PAGE)
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Cat. No.
BT43917
Source
Baculovirus
Synonyms
ORF9; Putative zinc-binding protein ORF9
Purity
>85% (SDS-PAGE)
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Cat. No.
BT43917
Source
Mammalian cell
Synonyms
ORF9; Putative zinc-binding protein ORF9
Purity
>85% (SDS-PAGE)
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Description

Overview of ORF9 in Ictalurid Herpesvirus 1 (IcHV-1)

Ictalurid herpesvirus 1 (IcHV-1), or channel catfish virus (CCV), is a member of the Alloherpesviridae family. Its 134 kbp genome encodes 79 putative open reading frames (ORFs), with viral proteins categorized into immediate-early (IE), early, and late kinetic classes based on temporal expression . While ORF9 is described as a putative zinc-binding protein in the query, no direct experimental data or peer-reviewed studies on recombinant ORF9 were identified in the provided search results. Below, we contextualize IcHV-1 zinc-binding proteins and recombinant protein research to infer potential characteristics of ORF9.

Zinc-Binding Proteins in IcHV-1

IcHV-1 encodes several proteins with zinc-binding motifs critical for viral replication and regulation:

ProteinKinetic ClassZinc-Binding MotifFunctionReferences
ORF12Immediate-earlyC3HC4 RING fingerTranscriptional regulation, ICP0 homolog
ORF59LateN/AEnvelope glycoprotein, viral entry
ORF19N/AN/APutative membrane protein

Key Observations:

  • ORF12, a well-characterized IE protein, contains a RING finger motif (C3HC4) involved in transcriptional regulation, analogous to ICP0 in herpes simplex virus .

  • Recombinant ORF59 (glycoprotein) inhibits viral entry when expressed in baculovirus systems .

  • ORF9’s absence in literature suggests it may be understudied or misannotated.

Recombinant Protein Expression in IcHV-1 Research

Recombinant proteins of IcHV-1 are typically generated for functional studies, vaccine development, or diagnostic tools. Examples include:

ORF59 Glycoprotein

  • Expression System: Baculovirus (Sf9 insect cells) with His6 tag .

  • Function: Blocks CCV entry via competitive inhibition .

  • Purity: >85% (SDS-PAGE) .

ORF19 Membrane Protein

  • Expression System: E. coli with N-terminal His tag .

  • Sequence: 204 amino acids, including transmembrane domains .

ORF12 (IE3C)

  • Role: Putative transcriptional regulator with RING finger motif .

Hypothetical Characterization of Recombinant ORF9

Based on IcHV-1 protein annotation practices and homologous systems:

Potential Applications

  • Diagnostics: Antigen for antibody production.

  • Functional Studies: Role in viral replication or immune evasion.

Research Gaps and Future Directions

  • Sequence Validation: Confirm ORF9’s existence and zinc-binding motif via genomic reannotation.

  • Expression Systems: Optimize E. coli or baculovirus platforms for soluble recombinant ORF9.

  • Functional Assays: Test DNA-binding capacity, subcellular localization, and impact on viral replication.

Product Specs

Form
Lyophilized powder. We will preferentially ship the available format. If you have special format requirements, please note them when ordering.
Lead Time
Delivery times vary based on purchasing method and location. Consult your local distributor for specific delivery times. All proteins are shipped with blue ice packs by default. Request dry ice shipment in advance (extra fees apply).
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer ingredients, storage temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
ORF9; Putative zinc-binding protein ORF9
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-158
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Ictalurid herpesvirus 1 (strain Auburn) (IcHV-1) (Channel catfish herpesvirus)
Target Names
ORF9
Target Protein Sequence
MATRPKVRRP DRALYVPPKV DRIKRVIFAC GHRKRCEIGY RPLGDSGNCG PCGARRDVFA PDLYGSGPLP PCPVCGRAVV GPTVREACGH VTCNACETEA CAVDRLCIGG GRRLVAICDP YPPYPGPRWR GPRPTRPEAH EAVQRSRGSS EDACTCAP
Uniprot No.
Q00163

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