Recombinant Klebsiella pneumoniae subsp. pneumoniae ATP synthase subunit b (atpF)

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Cat. No.
BT2054855
Source
in vitro E.coli expression system
Synonyms
atpF; KPN78578_41000; KPN_04141; ATP synthase subunit b; ATP synthase F(0 sector subunit b; ATPase subunit I; F-type ATPase subunit b; F-ATPase subunit b
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Description

Functional Role in K. pneumoniae

ATP synthase subunit b (atpF) is integral to the F₀F₁ ATP synthase complex, which catalyzes ATP production via oxidative phosphorylation. This enzyme enables bacterial adaptation to anaerobic conditions, including nitrogen fixation .

Key functional insights:

  • Energy Metabolism Regulation: Knockout studies (ΔompR mutants) show that atpF overexpression correlates with increased ATP production and reduced mucoviscosity, linking energy status to virulence .

  • Nitrogen Fixation: Approximately 30% of K. pneumoniae strains utilize ATP synthase for nitrogen fixation in anaerobic environments .

Immune Evasion Mechanisms

  • NF-κB Pathway Suppression: Wild-type K. pneumoniae uses atpF-associated ATP synthase activity to block NF-κB activation, a critical immune response pathway. Mutants lacking CPS (capsular polysaccharide) or LPS O-antigen show compromised evasion, triggering TLR2/TLR4-dependent inflammation .

  • Role in Virulence: Strains with intact ATP synthase exhibit enhanced survival in host tissues due to optimized energy metabolism and anti-inflammatory effects .

Hypermucoviscosity Regulation

Transcriptomic analyses reveal that atpF expression is inversely related to hypermucoviscosity. Overexpression of ATP synthase subunits reduces mucoviscosity by diverting metabolic resources toward ATP production rather than capsule synthesis .

Applications in Vaccine Development

Recombinant atpF is used as an antigenic target for vaccine candidates. Its conservation across Klebsiella strains and surface accessibility make it a promising immunogen .

ApplicationDetails
Antigen ProductionUsed to generate antibodies for preclinical studies
Immune Response StudiesEvaluated for eliciting T-cell and antibody-mediated responses
Commercial AvailabilityOffered by suppliers like Creative Biolabs and CUSABIO

Future Directions

Current research focuses on:

  • Therapeutic Targeting: Disrupting ATP synthase to sensitize K. pneumoniae to host immune responses .

  • Vaccine Optimization: Enhancing antigen presentation using recombinant atpF in combination with adjuvants .

Product Specs

Form
Lyophilized powder
Note: We prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it in your order notes, and we will fulfill your request.
Lead Time
Delivery time may vary depending on the purchase method and location. For specific delivery timelines, please consult your local distributors.
Note: All protein shipments are standardly packaged with blue ice packs. If you require dry ice shipment, please inform us in advance, as additional fees will apply.
Notes
Repeated freeze-thaw cycles are not recommended. For optimal preservation, store working aliquots at 4°C for up to one week.
Reconstitution
We recommend centrifuging the vial briefly before opening to ensure the contents settle at the bottom. Reconstitute the protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We suggest adding 5-50% glycerol (final concentration) and aliquotting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%. Customers can use this as a reference.
Shelf Life
Shelf life is influenced by various factors, including storage conditions, buffer composition, temperature, and the intrinsic stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is recommended for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
Tag type is established during production. If you have a specific tag requirement, please inform us, and we will prioritize developing the specified tag.
Synonyms
atpF; KPN78578_41000; KPN_04141; ATP synthase subunit b; ATP synthase F(0 sector subunit b; ATPase subunit I; F-type ATPase subunit b; F-ATPase subunit b
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-154
Protein Length
full length protein
Species
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Target Names
atpF
Target Protein Sequence
MNATILGQAIAFVIFVWFCMKYVWPPLMAAIEKRQKEISDGLASAERAKKDLDLAQANATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIVAQAQAEIDAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL
Uniprot No.
A6TG40

Target Background

Function
F(1)F(0) ATP synthase catalyzes the production of ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases comprise two structural domains: F(1), containing the extramembraneous catalytic core, and F(0), containing the membrane proton channel, linked by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled to proton translocation through a rotary mechanism involving the central stalk subunits. This subunit forms part of the peripheral stalk, connecting F(1) to F(0).
Database Links

KEGG: kpn:KPN_04141

STRING: 272620.KPN_04141

Protein Families
ATPase B chain family
Subcellular Location
Cell inner membrane; Single-pass membrane protein.

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