Recombinant Kocuria rhizophila ATP synthase subunit b (atpF)
Description
Introduction to ATP Synthase and Subunit b (atpF)
ATP synthase, also known as F1FO ATPase, is an essential enzyme that produces ATP (adenosine triphosphate) from ADP (adenosine diphosphate) using a proton or sodium gradient . It consists of two main structural domains: F1, which contains the extramembraneous catalytic core, and F0, which contains the membrane proton channel . These domains are linked by a central stalk and a peripheral stalk . Subunit b (atpF) is a component of the F0 domain .
Kocuria rhizophila: A Brief Overview
Kocuria rhizophila is a bacterium known for its diverse metabolic capabilities . Genomic analysis reveals its ability to synthesize amino acids like serine, cysteine, and aspartate, which can then be used to generate other essential amino acids such as valine and leucine .
Recombinant ATP Synthase Subunit b (atpF)
Recombinant ATP synthase subunit b (atpF) refers to the protein produced through recombinant DNA technology . This involves introducing the gene encoding the atpF subunit into a host organism (e.g., E. coli) and expressing it to produce the protein . The recombinant protein can then be isolated and purified for various research and industrial applications .
Recombinant Production: The process typically involves the following steps:
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Gene Cloning: The atpF gene from a source organism (Kocuria rhizophila) is isolated and cloned into a plasmid vector.
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Transformation: The plasmid is introduced into a host organism (E. coli).
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Expression: The host organism is cultured under conditions that promote expression of the atpF gene, leading to the production of the ATP synthase subunit b protein.
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Purification: The recombinant protein is then purified using techniques such as affinity chromatography, exploiting tags like His-tag fused to the protein .
Functional Significance and Research Applications
ATP synthase and its subunits, including subunit b (atpF), are critical for energy production in cells . Variations in subunit structures and functions can impact the efficiency and regulation of ATP synthesis .
Research Applications:
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Structural Studies: Recombinant atpF can be used to determine the structure and function of the ATP synthase complex .
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Drug Discovery: The ATP synthase enzyme is a target for several drugs, including TMC207, which binds to subunit c and inhibits ATP synthesis in Mycobacterium tuberculosis .
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Metabolic Engineering: Understanding the role of atpF in ATP synthesis can help in metabolic engineering to enhance ATP production in various organisms .
Available Recombinant atpF Products
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized fulfillment.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its inclusion.
Target Background
F1F0 ATP synthase synthesizes ATP from ADP using a proton or sodium gradient. This enzyme comprises two domains: the F1 catalytic core (extramembranous) and the F0 membrane proton channel. These domains are linked by a central and a peripheral stalk. ATP synthesis in the F1 catalytic domain is coupled, via a rotary mechanism of the central stalk subunits, to proton translocation. This protein is a component of the F0 channel, forming part of the peripheral stalk which connects F1 and F0.
KEGG: krh:KRH_09800
STRING: 378753.KRH_09800
