Recombinant Lactobacillus acidophilus Cysteine--tRNA ligase (cysS)
Description
Introduction to Recombinant Lactobacillus acidophilus Cysteine--tRNA ligase (cysS)
Recombinant Lactobacillus acidophilus Cysteine--tRNA ligase, encoded by the gene cysS, is an enzyme that plays a crucial role in the process of protein synthesis. This enzyme is responsible for attaching the amino acid cysteine to its corresponding transfer RNA (tRNA) molecule, which is essential for incorporating cysteine into proteins during translation. The recombinant form of this enzyme is produced through genetic engineering techniques, allowing for its large-scale production and purification for various applications in research and biotechnology.
Function and Mechanism
Cysteine--tRNA ligase, or cysteinyl-tRNA synthetase, catalyzes the reaction between cysteine and tRNA to form cysteinyl-tRNA. This process involves two main steps: the activation of cysteine to form an aminoacyl-adenylate intermediate, followed by the transfer of cysteine to tRNA, resulting in the formation of cysteinyl-tRNA. This enzyme is highly specific for cysteine and ensures that cysteine is accurately incorporated into proteins during translation.
Characteristics of Recombinant Lactobacillus acidophilus Cysteine--tRNA ligase
-
Purity: The recombinant enzyme is typically purified to a high degree, often greater than or equal to 85% purity as determined by SDS-PAGE .
-
Storage Conditions: For long-term storage, the enzyme should be conserved at -20°C .
-
Organism: The enzyme is derived from Lactobacillus acidophilus, a species commonly used in probiotics and known for its health benefits .
Data and Tables
Table 1: Characteristics of Recombinant Lactobacillus acidophilus Cysteine--tRNA ligase
| Characteristic | Description |
|---|---|
| Purity | ≥ 85% |
| Storage | -20°C |
| Organism | Lactobacillus acidophilus |
| Gene | cysS |
Table 2: General Functions of Cysteinyl-tRNA Synthetases
| Function | Description |
|---|---|
| Aminoacylation | Attaches cysteine to tRNA for protein synthesis |
| Specificity | High specificity for cysteine |
| Potential Roles Beyond Synthesis | May be involved in other cellular processes like polysulfide synthesis |
References https://www.nature.com/articles/s41467-017-01311-y https://pmc.ncbi.nlm.nih.gov/articles/PMC6407526/ https://pubmed.ncbi.nlm.nih.gov/8524650/ https://www.mybiosource.com/pathway/22518 https://www.uniprot.org/uniprotkb/Q5FM34/entry https://www.cusabio.com/datasheet/202041/EP-B/ https://www.uniprot.org/uniprot/Q74L26 https://www.tandfonline.com/doi/pdf/10.4161/rna.1.1.910
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type is determined during production. If you require a specific tag type, please inform us, and we will prioritize its development.
Target Background
KEGG: lac:LBA0348
STRING: 272621.LBA0348
