Recombinant Lactobacillus acidophilus Probable DNA-directed RNA polymerase subunit delta (rpoE)
Description
Introduction to Recombinant Lactobacillus acidophilus Probable DNA-directed RNA Polymerase Subunit Delta (rpoE)
The recombinant Lactobacillus acidophilus probable DNA-directed RNA polymerase subunit delta (rpoE) is a genetically engineered version of the delta subunit of RNA polymerase found in Lactobacillus acidophilus. This subunit is crucial for enhancing transcriptional specificity and efficiency in bacteria. While specific research on the recombinant version of rpoE in Lactobacillus acidophilus is limited, understanding its role can be inferred from studies on similar subunits in other bacteria.
Role of RNA Polymerase Subunit Delta (rpoE) in Bacteria
In bacteria, RNA polymerase is the enzyme responsible for transcribing DNA into RNA. The delta subunit (rpoE) is particularly important in low-G+C Gram-positive bacteria, such as Lactobacillus acidophilus, where it enhances transcriptional specificity by blocking RNA polymerase binding at weak promoter sites and stimulating RNA synthesis by accelerating core enzyme recycling .
Functions of rpoE in Bacteria
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Transcriptional Specificity: rpoE helps in preventing non-specific binding of RNA polymerase to DNA, ensuring that transcription is initiated at the correct sites .
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RNA Synthesis Efficiency: By facilitating the recycling of the RNA polymerase core enzyme, rpoE increases the efficiency of RNA synthesis .
Potential Applications of Recombinant rpoE
While direct applications of recombinant Lactobacillus acidophilus rpoE are not well-documented, enhancing transcriptional efficiency could potentially improve the production of beneficial compounds by Lactobacillus acidophilus, such as exopolysaccharides (EPS) or other probiotic factors .
Product Specs
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Target Background
This protein participates in both the initiation and recycling phases of transcription. In the presence of the delta subunit, RNA polymerase displays enhanced transcription specificity, reduced nucleic acid affinity, and improved RNA synthesis efficiency due to more efficient recycling.
KEGG: lac:LBA0232
STRING: 272621.LBA0232
