Recombinant Lactobacillus johnsonii 50S ribosomal protein L17 (rplQ)
Description
Definition and Biological Significance
rplQ encodes the 50S ribosomal protein L17, essential for ribosomal assembly and function in bacteria. In Lactobacillus johnsonii, a probiotic species with demonstrated bile resistance, stress response, and host-adapted properties , ribosomal proteins like L17 may contribute to survival under gastrointestinal conditions. Recombinant production enables structural, functional, and therapeutic studies, particularly for understanding ribosomal dynamics and antimicrobial resistance .
Ribosomal Function
The 50S ribosomal subunit, including L17, facilitates peptide bond formation and tRNA accommodation. Structural models (e.g., AlphaFold DB for Campylobacter jejuni L17 ) suggest L17 adopts a globular fold, interacting with rRNA and stabilizing the peptidyl transferase center. While L. johnsonii-specific structures are unavailable, homologous models provide a foundation for studying its role in translation fidelity and antibiotic resistance .
Stress Response and Antibiotic Resistance
In L. johnsonii, ribosomal proteins may modulate resistance to bile salts and antibiotics. For example:
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Bile Resistance: Proteomic studies show upregulation of ribosomal proteins under bile stress, indicating their role in maintaining translation efficiency during environmental challenges .
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Antibiotic Targeting: rplQ homologs in other species (e.g., Streptococcus) are linked to macrolide resistance via ribosomal methylation (e.g., ermB genes) .
Experimental Use Cases
Recombinant rplQ is employed in:
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Structural Biology: Crystallography or cryo-EM studies to resolve ribosomal interactions.
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Antimicrobial Studies: Testing interactions with antibiotics like macrolides or lincosamides.
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Probiotic Research: Investigating ribosomal adaptation in L. johnsonii strains with enhanced gut persistence .
Limitations and Gaps
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Species-Specific Data: Most rplQ studies pertain to non-L. johnsonii species (e.g., Campylobacter , Streptococcus ).
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Functional Studies: Direct evidence linking L. johnsonii rplQ to probiotic properties (e.g., bile resistance, immune modulation) remains sparse .
Sequence and Functional Conservation
The amino acid sequence of L. johnsonii rplQ (hypothetical) overlaps with bacterial homologs. For example:
Note: Sequence data for L. johnsonii rplQ is inferred due to limited direct annotation.
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference in your order notes for customized preparation.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
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