Recombinant Lactobacillus johnsonii ATP synthase subunit b (atpF)
Description
Overview of Recombinant Lactobacillus johnsonii ATP Synthase Subunit b (atpF)
Recombinant Lactobacillus johnsonii ATP synthase subunit b (atpF) refers to a genetically engineered form of the AtpF protein, which is a subunit of the ATP synthase enzyme found in the bacterium Lactobacillus johnsonii . ATP synthase is an essential enzyme that produces adenosine triphosphate (ATP), the primary energy currency of cells . The "recombinant" aspect indicates that the gene encoding the AtpF subunit has been isolated, modified, and then expressed in a host organism, such as E. coli, to produce large quantities of the protein .
Characteristics
Production and Structure
The recombinant AtpF subunit is produced by cloning the atpF gene from Lactobacillus johnsonii into an expression vector, which is then introduced into E. coli . The E. coli cells are cultured to express the protein, and the recombinant AtpF subunit is then purified using affinity chromatography, often utilizing a His tag that is fused to the N-terminus of the protein .
Function and Biological Role
The ATP synthase enzyme is crucial for energy production in bacteria, catalyzing the synthesis of ATP from adenosine diphosphate (ADP) and inorganic phosphate using the proton motive force generated across the cell membrane . Subunit b (AtpF) is a critical component of the F0 sector of the ATP synthase, which is embedded in the membrane and responsible for proton translocation.
Potential Applications
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Antimicrobials Lactobacillus produces diverse metabolites, such as peptides, that have antimicrobial properties .
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Functional Foods: Fermenting food with Lactobacillus enhances the food's qualities, and may lead to the development of functional foods .
Research and Studies
Lactobacillus johnsonii is a probiotic bacterium with several beneficial effects, including inhibiting pathogenic bacteria and improving antioxidant activity . Studies have explored the genetic and metabolic characteristics of Lactobacillus johnsonii strains to understand their probiotic properties and mechanisms of action . Recombinant enzymes from Lactobacillus johnsonii have demonstrated anti-giardial activity, suggesting potential therapeutic applications .
Product Specs
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The specific tag will be determined during production. If you require a specific tag, please inform us for preferential development.
Target Background
KEGG: ljo:LJ_0936
STRING: 257314.LJ0936
