Recombinant Lactobacillus johnsonii Glutamate--tRNA ligase (gltX)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder.
Note: While we will prioritize shipping the format currently in stock, please specify your format preference during order placement for customized fulfillment.
Note: While we will prioritize shipping the format currently in stock, please specify your format preference during order placement for customized fulfillment.
Lead Time
Delivery times vary depending on the purchase method and location. Please contact your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is requested. Advance notice and additional charges apply for dry ice shipping.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is requested. Advance notice and additional charges apply for dry ice shipping.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to settle the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a guideline.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer components, temperature, and the protein's inherent stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process.
Note: If you require a specific tag type, please inform us, and we will prioritize its inclusion in the production process.
Note: If you require a specific tag type, please inform us, and we will prioritize its inclusion in the production process.
Synonyms
gltX; LJ_0399; Glutamate--tRNA ligase; EC 6.1.1.17; Glutamyl-tRNA synthetase; GluRS
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-499
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Target Names
gltX
Target Protein Sequence
MAKQKIRVRY APSPTGHLHI GNARTALFNY LFARHNKGTM VLRIEDTDQK RNVEGGSKSQ
MENLHWLGID WDEGPDKGGD FGPYRQSERK DIYNKYIQQL IDEGKAYYSY KTEEELEAQR
EEQRAMGIAP HYTYEYEGMT ADEIKEAQAA AEAKGLKPVV RIHIPENRTY AWEDMVKGKV
SFESDTIGGD FVIQKRDGMP TYNFAVVIDD HLMEITHVLR GDDHVANTPK QLVVYEALGW
EPPKFGHMTL IINSETGKKL SKRDESVLQF IEQYRDLGYL PDAMFNFITL LGWSPVGESE
IFSQRELIKS FDPKRLSKSP AAFDQKKLEW INNQYVKKAD RDLLLDLALN NLQEAGLVDK
EISPEKMEWV RQLVNIYAVQ MSYTKQIVDI AKIFFEEAPE LSDAEIEEIK KDDARPVIEE
FKKQLNAVPR FTAVQAMNAI QATRRETGVK GRKLFMPIRI AATRSMVGPG IGEAIELMGK
EKVNKHIDLT LKQLSDLGL
Uniprot No.
Target Background
Function
This enzyme catalyzes the two-step attachment of glutamate to tRNA(Glu): glutamate is first activated by ATP to form Glu-AMP, which is then transferred to the acceptor end of tRNA(Glu).
Database Links
KEGG: ljo:LJ_0399
STRING: 257314.LJ0399
Protein Families
Class-I aminoacyl-tRNA synthetase family, Glutamate--tRNA ligase type 1 subfamily
Subcellular Location
Cytoplasm.
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