Recombinant Lactobacillus johnsonii Guanylate kinase (gmk)
Shipped with Ice Packs 
In Stock 
Description
Guanylate Kinase (GMK) in Bacterial Physiology
GMK catalyzes the ATP-dependent phosphorylation of GMP to GDP, a key step in GTP biosynthesis. Its regulation by stress-signaling nucleotides like (p)ppGpp has been extensively studied:
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Mechanism of Action:
Recombinant Lactobacillus johnsonii Engineering
Though no studies explicitly describe GMK engineering in L. johnsonii, its genetic tractability and probiotic properties make it a candidate for metabolic engineering:
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Genetic Tools for *L. johnsonii*:
| Parameter | Details |
|---|---|
| Vector | pPG612 (chloramphenicol resistance) |
| Induction method | Xylose (10 g/L) |
| Protein detection | 15 kDa band (GM-CSF) |
| Genetic stability | Maintained over 40 generations |
Potential Applications of Engineered GMK in L. johnsonii
Hypothetical applications based on GMK’s role in other bacteria:
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Metabolic Engineering:
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Therapeutic Development:
Research Gaps and Future Directions
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Unanswered Questions:
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Endogenous GMK activity in L. johnsonii remains uncharacterized.
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No studies have reported GMK overexpression or knockout in this species.
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Opportunities:
Product Specs
Form
Lyophilized powder. We will ship the in-stock format preferentially. If you have special format requirements, please note them when ordering.
Lead Time
Delivery time varies by purchase method and location. Consult local distributors for specific delivery times. All proteins are shipped with standard blue ice packs. Request dry ice in advance for an additional fee.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots are stable at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
gmk; LJ_1543; Guanylate kinase; EC 2.7.4.8; GMP kinase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-204
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)
Target Names
gmk
Target Protein Sequence
MAHQGLLLVL SGPSGVGKGT VKSAMVKQKA FSFEYSVSMT TRKPRPGEVN GKDYYFVSED
RFQEAIKGNE LLEYNEYVGN HYGTPLAPVQ KMLNEGKDVL LEIDVNGAKQ VRKLMPDGVF
IFLTPPDLHE LKHRIVNRGT DSDKVIAMRM KQARKEILMM EDYDYAVVND TVANAVDHIK
SIVEAEHVRV PRVINDYRNM VKED
Uniprot No.
Target Background
Function
Essential for recycling GMP and, indirectly, cGMP.
Database Links
KEGG: ljo:LJ_1543
STRING: 257314.LJ1543
Protein Families
Guanylate kinase family
Subcellular Location
Cytoplasm.
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