Recombinant Lactobacillus plantarum Acylphosphatase (acyP)
Description
Characteristics of Acylphosphatase
Lactobacillus plantarum is a lactic acid bacterium with the ability to express enzymes with therapeutic potential when produced recombinantly . Acylphosphatases (AcyPs) are small, highly conserved enzymes that catalyze the hydrolysis of acylphosphates .
ACYP1 Protein (Human) Characteristics :
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Catalog number: B2011385
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Lot number: Batch Dependent
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Expiration Date: Batch dependent
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Amount: 100 µg
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Molecular Weight or Concentration: 13.6 kDa
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Supplied as: Solution
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Applications: molecular tool for various biochemical applications
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Storage: -20 °C
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Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.
Applications in Immunological Research
Recombinant L. plantarum can be engineered to express and deliver antigens, enhancing immune responses. For instance, recombinant L. plantarum expressing the H7N9 antigen has been shown to induce IgA expression in the lungs and intestines of mice, with the HA1-DCpep group demonstrating a more pronounced advantage .
Metabolic Effects
Research indicates that Lactobacillus plantarum influences metabolic pathways. For example, in Streptococcus bovis, inactivation of ccpA affects glycolysis and pyruvate metabolism, with genes encoding acetyl-CoA carboxylase biotin and acyl phosphatase being downregulated in the ccpA mutant .
Treatment of Hypertension
Recombinant Lactobacillus plantarum expressing angiotensin-converting enzyme inhibitory peptides (ACEIPs) has demonstrated effectiveness in treating hypertension in spontaneously hypertensive rats (SHRs) . Oral administration of recombinant Lb. plantarum NC8 significantly decreased systolic blood pressure, increased nitric oxide levels, and decreased endothelin and angiotensin II levels . The following table displays the effects on hematology and blood biochemistry:
Table 1. Effects on hematology and blood biochemistry
| Parameters | PBS | NC8 | RLP |
|---|---|---|---|
| WBC (10 9/L) | 5.133 ± 1.935 | 5.567 ± 0.929 | 5.400 ± 2.272 |
| RBC (10 12/L) | 6.810 ± 0.764 | 6.727 ± 0.748 | 7.620 ± 0.735 |
| HGB (g/L) | 94.33 ± 14.154 | 102.33 ± 6.658 | 113.00 ± 10.583 |
| HCT (%) | 0.308 ± 0.013 | 0.320 ± 0.047 | 0.382 ± 0.034* |
| MCV (fL) | 50.500 ± 0.700 | 50.000 ± 1.200 | 50.133 ± 0.351 |
| MCH (pg) | 14.133 ± 0.902 | 15.667 ± 0.757 | 14.833 ± 0.058 |
| MCHC (g/L) | 296.00 ± 30.610 | 314.00 ± 22.113 | 295.67 ± 1.155 |
| PLT (10 9/L) | 533.33 ± 59.181 | 589.33 ± 36.611 | 661.67 ± 289.096 |
| W-SCR (%) | 0.752 ± 0.064 | 0.607 ± 0.132 | 0.793 ± 0.159 |
| W-SCC (%) | 3.000 ± 0.600 | 3.133 ± 0.231 | 4.400 ± 2.193 |
| RDW-SD (fL) | 29.000 ± 0.100 | 29.033 ± 0.379 | 28.800 ± 0.625 |
| RDW-CV (%) | 0.147 ± 0.001 | 3.147 ± 5.198 | 0.148 ± 0.009 |
| PDW (fL) | 7.500 ± 0.100 | 7.733 ± 0.451 | 7.967 ± 0.643 |
| MPV (fL) | 6.200 ± 0.100 | 6.433 ± 0.231 | 6.700 ± 0.361 |
| P-LCR (%) | 0.030 ± 0.011 | 0.042 ± 0.009 | 0.047 ± 0.013 |
| UREA (mmol/L) | 8.343 ± 1.517 | 8.340 ± 0.963 | 7.700 ± 1.908 |
| GLUC (mmol/L) | 16.570 ± 2.370 | 14.300 ± 2.383 | 15.480 ± 1.019 |
| TCHE (mmol/L) | 1.360 ± 0.044 | 1.333 ± 0.125 | 1.100 ± 0.050* ,# |
| TG (mmol/L) | 1.247 ± 0.025 | 1.213 ± 0.176 | 0.750 ± 0.181* ,# |
*P < 0.05 compared with the PBS control group.
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for custom preparation.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional fees.
If you require a specific tag, please inform us; we will prioritize its development.
