Recombinant Lactobacillus plantarum DNA translocase FtsK (ftsK)
Description
Key Functional Properties
Recombinant Production and Characterization
The recombinant L. plantarum FtsK is produced as a full-length (1–795 amino acids) His-tagged protein in E. coli (see Table 1) .
Genome Segregation and Cell Division
FtsK coordinates chromosome segregation with cell division in both single- and multipartite genome bacteria:
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Chromosome dimer resolution (CDR): Translocates DNA to resolve dimers via XerCD-mediated recombination at the dif site .
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Multipartite genome management: In Deinococcus radiodurans, FtsK aligns foci at septa and nucleoids during division, ensuring accurate segregation of polyploid genomes .
Stress Response and Proteolytic Regulation
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Heat shock regulation: L. plantarum ftsK is transcriptionally induced under heat stress, controlled by the CtsR repressor .
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Proteolytic regulation: In Bacillus subtilis, FtsK modulates Sle1 peptidoglycan hydrolase levels, linking DNA translocation to cell wall remodeling .
Comparative Analysis Across Species
FtsK homologs share conserved structural motifs but exhibit species-specific adaptations (Table 2).
Table 2: FtsK Homologs in Gram-Positive Bacteria
Experimental Challenges and Future Directions
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Domain-specific studies: Deletion mutants of L. plantarum FtsK (e.g., Δγ domain) alter membrane architecture and nucleoid morphology, highlighting its multifunctional role .
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Radiation response: D. radiodurans FtsK upregulates post-irradiation, suggesting roles in DNA repair and genome reorganization .
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them in your order. We will fulfill your request whenever possible.
Note: Our proteins are standardly shipped with normal blue ice packs. If you require dry ice shipping, please inform us in advance as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: lpl:lp_2210
STRING: 220668.lp_2210
