Recombinant Lactococcus lactis subsp. lactis Lipoprotein signal peptidase (lspA) (I65V)
Description
Introduction
Recombinant Lactococcus lactis subsp. lactis lipoprotein signal peptidase (lspA) (I65V) is a genetically engineered variant of the lspA enzyme, which plays a critical role in bacterial lipoprotein processing. This enzyme catalyzes the cleavage of signal peptides from prolipoproteins, an essential step in bacterial membrane protein maturation . The I65V mutation (isoleucine to valine substitution at position 65) introduces structural and functional modifications to the enzyme, though its specific biochemical implications require further characterization.
Molecular Architecture
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Gene: Encoded by the lspA gene (locus LL0997/L0335 in strain IL1403) .
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Catalytic Activity: Functions as signal peptidase II (EC 3.4.23.36), targeting lipoproteins for membrane anchoring .
Recombinant Expression
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Host System: Typically expressed in Escherichia coli or L. lactis expression systems .
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Tags: Includes affinity tags (e.g., His-tag) for purification, though the exact tag varies by production protocol .
Role in Bacterial Physiology
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Lipoprotein Processing: Essential for virulence in gram-positive bacteria, making lspA a potential antimicrobial target .
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Nanodisc Integration: The I65V variant has been stabilized in nanodiscs for structural studies, enabling analysis of membrane-protein interactions .
Biotechnological Relevance
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Probiotic Engineering: L. lactis is widely used for recombinant protein delivery (e.g., HSP65, cytokines) . While lspA itself is not a therapeutic agent, its study informs optimization of secretion systems in engineered strains.
Supplier Data
| Supplier | Location | Product Code | Purity |
|---|---|---|---|
| CUSABIO TECHNOLOGY | China | CSB-CF875000LNG | ≥90% |
Research Gaps and Future Directions
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have a specific format preference, please indicate it in your order notes, and we will accommodate your request.
Note: All protein shipments are standardly packaged with blue ice packs. If dry ice packaging is required, please contact us in advance, as additional fees will apply.
Generally, liquid form has a shelf life of 6 months at -20°C/-80°C. Lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Target Background
KEGG: lla:L0335
STRING: 272623.L0335
