Recombinant Lactococcus lactis subsp. cremoris Alkaline phosphatase-like protein (apl)
Description
Molecular Characterization
Recombinant Lactococcus lactis subsp. cremoris Alkaline Phosphatase-Like Protein (Apl) is a 214-amino-acid protein (UniProt ID: Q48630) produced through heterologous expression in Escherichia coli systems . Key molecular features include:
The protein shares homology with alkaline phosphatases but exhibits unique structural adaptations for microbial environments, including transmembrane domains inferred from its hydrophobic sequence .
Expression Systems
While traditionally produced in E. coli , recent advances utilize L. lactis subsp. cremoris for LPS-free production. Key protocols include:
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Aggregate-based purification: Solubilization of functional protein aggregates (inclusion bodies) using mild detergents, achieving >98.5% purity .
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Cost-effective workflow: Combines cell lysis, aggregate isolation, and refolding without denaturing agents .
Biochemical Properties
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Activity: Retains phosphatase-like functionality, though substrate specificity remains under investigation .
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Thermostability: Optimal activity at pH 7–8 and temperatures up to 40°C .
Industrial Relevance
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Biocatalysis: Potential for phosphate ester hydrolysis in dairy fermentation processes .
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Bioremediation: Explored for enzymatic degradation of organic phosphates in wastewater .
Immunological Studies
While not directly linked to Apl, L. cremoris strains like YRC3780 demonstrate immunomodulatory effects via dendritic cell-mediated Treg induction . This highlights the broader biotechnological value of L. cremoris-derived proteins.
Advantages Over Conventional Systems
Challenges and Future Directions
Product Specs
Please note: We will prioritize shipping the format currently in stock. However, if you have a specific format preference, please specify it in your order notes. We will fulfill your request as best as possible.
Important: All proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please inform us in advance, as additional fees will apply.
Generally, liquid formulations have a shelf life of 6 months at -20°C/-80°C. Lyophilized forms have a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: llm:llmg_1854
STRING: 416870.llmg_1854
