Recombinant Lactococcus lactis subsp. lactis Alpha-acetolactate decarboxylase (aldB)

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Cat. No.
BT35817
Source
Yeast
Synonyms
aldB; LL1228; L0321Alpha-acetolactate decarboxylase; EC 4.1.1.5
Purity
>85% (SDS-PAGE)
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Cat. No.
BT35817
Source
E.coli
Synonyms
aldB; LL1228; L0321Alpha-acetolactate decarboxylase; EC 4.1.1.5
Purity
>85% (SDS-PAGE)
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Cat. No.
BT35817
Source
E.coli
Synonyms
aldB; LL1228; L0321Alpha-acetolactate decarboxylase; EC 4.1.1.5
Purity
>85% (SDS-PAGE)
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Cat. No.
BT35817
Source
Baculovirus
Synonyms
aldB; LL1228; L0321Alpha-acetolactate decarboxylase; EC 4.1.1.5
Purity
>85% (SDS-PAGE)
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Cat. No.
BT35817
Source
Mammalian cell
Synonyms
aldB; LL1228; L0321Alpha-acetolactate decarboxylase; EC 4.1.1.5
Purity
>85% (SDS-PAGE)
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Description

Genetic and Functional Organization

The aldB gene in L. lactis is clustered with BCAA biosynthesis genes (ilvBN) and exhibits dual transcriptional control:

  • BCAA-dependent regulation: Transcribed alongside ilvBN under isoleucine-mediated attenuation .

  • Independent regulation: Activated by its own promoter during catabolic acetolactate synthesis .

The enzyme catalyzes the decarboxylation of α-acetolactate to acetoin, a precursor for diacetyl (2,3-butanedione), and modulates acetolactate flux between biosynthesis and catabolism .

Enzymatic Properties

PropertyDescriptionCitation
Subunit structureComposed of six identical 26.5 kDa subunits
Allosteric activationActivated by leucine, valine, and isoleucine (allosteric effectors)
Kinetic parametersMichaelis-Menten kinetics in leucine presence; allosteric behavior otherwise
StabilityUnstable in cell extracts; enhanced expression in E. coli systems

Metabolic Engineering Applications

Recombinant aldB strains are engineered for:

  • Diacetyl production: Deletion of aldB redirects acetolactate to diacetyl via spontaneous decarboxylation .

  • Flavor enhancement: Overexpression of ilvBN in aldB-deleted strains amplifies acetolactate synthesis, boosting diacetyl yields .

Strain ModificationMetabolite LevelsCitation
aldB deletion (strain FI8076)Acetolactate: 0.53 mM; Acetoin: 0.48 mM
ilvBN overexpression + aldB deletionDiacetyl: 0.84 mM (10-fold increase)
NADH-oxidase overexpressionAcetoin: 80% glucose conversion to acetoin

Research Findings and Biotechnological Implications

  • Fermentation optimization: Engineered L. lactis strains achieve 80% pyruvate flux redirection to acetolactate, surpassing natural diacetylactis strains .

  • Biosensor integration: aldB-deficient strains enable real-time monitoring of diacetyl via GFP-based biosensors .

  • Gut microbiome modulation: Recombinant aldB-expressing L. lactis mitigates colitis by regulating pro-inflammatory cytokines (e.g., TNF-α, IFN-γ) .

Product Specs

Form
Lyophilized powder. Note: We will prioritize shipping the format we have in stock. If you have special format requirements, please specify them when ordering, and we will fulfill your request.
Lead Time
Delivery times vary based on purchasing method and location. Please contact your local distributor for specific delivery information. Note: All proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance as additional charges apply.
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. Adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C is recommended. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on several factors, including storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you require a specific tag type, please inform us, and we will prioritize developing it.
Synonyms
aldB; LL1228; L0321Alpha-acetolactate decarboxylase; EC 4.1.1.5
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-236
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Target Names
aldB
Target Protein Sequence
MTEITQLFQY NTLGALMAGL YEGTMTIGEL LKHGDLGIGT LDSVDGELIV LDGKAYQAKG DKTIVELTDD IKVPYAAVVP HQAEVVFKQK FTASDKELED RIESYFDGQN LFRSIKITGE FPKMHVRMIP RAKSGTRFVE VSQNQPEYTE ENVKGTIVGI WTPEMFHGVS VAGYHLHFIS EDFTFGGHVL DFIIDNGTVE IGAIDQLNQS FPVQDRKFLF ADLDIEALKK DIDVAE
Uniprot No.
P95676

Target Background

Function
Converts acetolactate into acetoin.
Database Links

KEGG: lla:L0321

STRING: 272623.L0321

Protein Families
Alpha-acetolactate decarboxylase family

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