Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Apolipoprotein N-acyltransferase 1 (lnt1)
Description
Gene and Protein Overview
lnt1 is encoded by the lnt1 gene (also referred to as LIC_1124 or LA_1124 in genomic databases). It functions as an acyltransferase, potentially involved in modifying bacterial lipoproteins or surface components . The protein is expressed in recombinant formats, including full-length and partial variants, depending on the host system used.
Key Attributes:
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Gene Synonyms: lnt1, LIC_1124, LA_1124
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Protein Function: Apolipoprotein N-acyltransferase activity, likely involved in lipid metabolism or pathogen-host interactions.
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Pathogenic Relevance: Expressed in virulent strains of L. interrogans serovar Copenhageni, a highly virulent serovar within serogroup Icterohaemorrhagiae .
Production and Recombinant Forms
lnt1 is produced via heterologous expression in diverse host systems, with variations in protein length and host compatibility. Below is a comparative analysis of recombinant lnt1 variants:
| Variant | Host System | Purity | Serovar | Protein Length |
|---|---|---|---|---|
| Full-length lnt1 | Cell-free expression | ≥85% (SDS-PAGE) | Copenhageni | Full-length |
| Partial lnt1 (Copenhageni) | E. coli, Yeast, Baculovirus, Mammalian Cell | ≥85% (SDS-PAGE) | Copenhageni | Partial |
| Partial lnt1 (Lai) | E. coli, Yeast, Baculovirus, Mammalian Cell | ≥85% (SDS-PAGE) | Lai | Partial |
Notes:
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Full-Length vs. Partial: Full-length versions preserve the entire amino acid sequence, while partial variants exclude specific regions (e.g., N-terminal or C-terminal domains) .
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Host System Impact: Cell-free systems enable rapid production without host contamination, whereas E. coli and mammalian cells are used for scalable or post-translational modification purposes .
Applications in Research and Diagnostics
Recombinant lnt1 is primarily used in biochemical and immunological assays:
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Protein Purification: Served as a control in studies analyzing Leptospira protein interactions (e.g., LRR-protein binding assays) .
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Antigen Production: Potential utility in developing serogroup-specific diagnostic tools, though no validated assays are reported .
Comparative Genomic Context
Genomic analyses of L. interrogans serovar Copenhageni reveal distinct features:
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Conservation in Pathogenic Strains: lnt1 is absent in saprophytic Leptospira species (e.g., L. biflexa), highlighting its association with pathogenicity .
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Divergence from Serovar Icterohaemorrhagiae: A frameshift mutation in lic12008 (LPS biosynthesis gene) distinguishes serovar Icterohaemorrhagiae from Copenhageni, though lnt1 remains conserved .
Product Specs
Note: While we prioritize shipping the format currently in stock, we are happy to accommodate specific format requirements. Please indicate your preferred format during order placement, and we will do our best to fulfill your request.
Note: All protein shipments are standardly accompanied by normal blue ice packs. If dry ice shipping is required, please communicate with us in advance as additional fees will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. Lyophilized form, when stored under the same conditions, exhibits a shelf life of 12 months.
Target Background
KEGG: lic:LIC_12556
STRING: 267671.LIC12556
