Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni ATP synthase subunit b (atpF)
Description
Introduction to Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni ATP Synthase Subunit b (atpF)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni ATP synthase subunit b (atpF) is a recombinant protein derived from the bacterium Leptospira interrogans, specifically from the serovar copenhageni within the Icterohaemorrhagiae serogroup. This serogroup is known for its high virulence and is a major cause of severe leptospirosis cases worldwide . The ATP synthase subunit b (atpF) plays a crucial role in the energy production of Leptospira by facilitating the synthesis of ATP, which is essential for bacterial survival and pathogenicity.
Structure and Function of ATP Synthase Subunit b (atpF)
ATP synthase is an enzyme that uses the energy from a proton gradient across a membrane to drive the phosphorylation of ADP to ATP. The subunit b (atpF) is part of the F0 sector of this enzyme, which spans the membrane and is involved in the proton translocation process . The recombinant form of this protein is produced through genetic engineering techniques, allowing for its expression in host organisms like Escherichia coli.
Protein Details:
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Protein Name: ATP synthase subunit b
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Alternative Names: ATP synthase F(0) sector subunit b, ATPase subunit I, F-type ATPase subunit b
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Gene Name: atpF
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Ordered Locus Name: LIC_11239
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Sequence Length: 173 amino acids
Research Findings and Applications
Research on recombinant proteins like ATP synthase subunit b (atpF) from Leptospira is crucial for understanding the pathogenic mechanisms of the bacterium and for developing diagnostic tools or vaccines. The genetic diversity among different serovars, including copenhageni and Icterohaemorrhagiae, highlights the complexity of leptospirosis and the need for targeted therapeutic approaches .
Table: Key Features of Recombinant ATP Synthase Subunit b (atpF)
| Feature | Description |
|---|---|
| Protein Type | Recombinant protein |
| Species | Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni |
| Function | Essential for ATP synthesis in bacteria |
| Expression Host | Typically Escherichia coli |
| Storage Conditions | -20°C or -80°C for long-term storage |
Diagnostic and Therapeutic Potential
The recombinant ATP synthase subunit b (atpF) can be used in ELISA assays for detecting antibodies against Leptospira in infected individuals, aiding in the diagnosis of leptospirosis . Additionally, understanding the role of this protein in bacterial energy metabolism may lead to the development of novel therapeutic targets.
Table: Potential Applications
| Application | Description |
|---|---|
| Diagnostic Tool | ELISA assays for detecting antibodies against Leptospira |
| Therapeutic Target | Potential target for developing drugs that inhibit bacterial ATP synthesis |
Product Specs
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Target Background
F1F0 ATP synthase synthesizes ATP from ADP using a proton or sodium gradient. This enzyme comprises two domains: the F1 domain, containing the extramembrane catalytic core; and the F0 domain, containing the membrane proton channel. These domains are linked by a central and peripheral stalk. ATP synthesis in the F1 catalytic domain is coupled to proton translocation via a rotary mechanism involving the central stalk subunits. This protein is a component of the F0 channel, forming part of the peripheral stalk and linking F1 to F0.
KEGG: lic:LIC_11239
STRING: 267671.LIC11239
