Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni UDP-N-acetylenolpyruvoylglucosamine reductase (murB)
Description
Introduction to UDP-N-acetylenolpyruvoylglucosamine Reductase (murB)
UDP-N-acetylenolpyruvoylglucosamine reductase, encoded by the gene murB, is a crucial enzyme in the peptidoglycan biosynthesis pathway of bacteria. It catalyzes the reduction of UDP-N-acetylenolpyruvoylglucosamine to UDP-N-acetylmuramic acid, a key step in forming the bacterial cell wall . In Leptospira interrogans, particularly the serogroup Icterohaemorrhagiae serovar Copenhageni, this enzyme plays a vital role in maintaining the structural integrity of the cell wall.
Role in Peptidoglycan Biosynthesis
Peptidoglycan, also known as murein, is essential for bacterial cell wall structure, providing rigidity and strength necessary for cell growth and division . The biosynthesis pathway involves several enzymes, including MurA, MurB, MurC, MurD, MurE, and MurF. MurB specifically reduces the enolpyruvyl moiety of UDP-N-acetylenolpyruvoylglucosamine to form UDP-N-acetylmuramic acid, using NADPH as a cofactor .
Recombinant Expression and Research Significance
Recombinant expression of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni UDP-N-acetylenolpyruvoylglucosamine reductase (murB) involves cloning the murB gene into an expression vector and expressing it in a suitable host organism. This approach allows for large-scale production of the enzyme for biochemical studies and potential applications in drug development. Given the importance of peptidoglycan in bacterial survival, enzymes like murB are attractive targets for antibacterial drugs .
Research Findings and Data
While specific research findings on the recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni UDP-N-acetylenolpyruvoylglucosamine reductase (murB) are scarce, studies on related enzymes and serovars provide valuable insights:
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Note: Our proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
The tag type will be determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
KEGG: lic:LIC_13138
STRING: 267671.LIC13138
