Recombinant Lettuce infectious yellows virus Protein p59, partial
Description
Introduction to Recombinant Lettuce Infectious Yellows Virus Protein p59, Partial
Lettuce infectious yellows virus (LIYV) is a bipartite, single-stranded, positive-sense RNA virus in the family Closteroviridae and the type member of the genus Crinivirus . LIYV is transmitted by the sweet potato whitefly (Bemisia tabaci) . Virions of LIYV contain the major capsid protein (CP), the minor capsid protein (CPm), a 59 kDa protein (p59), and the HSP70 homologue . The LIYV genome consists of two RNA molecules, RNA1 and RNA2, which are separately encapsidated in flexuous filamentous particles .
Characteristics of p59 Protein
LIYV virions contain four protein components: the major coat protein (CP), the minor coat protein (CPm), an Hsp70 homolog (Hsp70h), and a 59-kDa protein (P59) . P59 and the HSP70 homologue were not detected on virions by immunogold labeling, but were always detected in virion preparations by immunoblot analysis .
Role of p59 in LIYV
The precise location of P59 within LIYV virions has not been identified, because it is not readily detected by immunogold labeling and transmission electron microscopy (IGL-TEM) . Studies of Citrus tristeza virus (CTV) and Beet yellows virus (BYV) have shown that genomic RNA encapsidation involves a complex interaction between Hsp70h, P61 (the homolog of P59 in CTV)/P64 (the homolog of P59 in BYV), and the CPm, and the interaction occurs near the 5′ end of the genomic RNA . It is possible that similar interactions occur for LIYV .
Experimental Analysis of LIYV p59
Virion preparations from LIYV-infected plants were analyzed using SDS-PAGE and immunoblotting . The results identified the major capsid protein (CP), minor capsid protein (CPm), p59, and the HSP70 homologue . Immunogold labeling showed that CP constituted most of the LIYV virion capsid, while CPm was found at one end of the virion . P59 and the HSP70 homologue were not detected on virions by immunogold labeling but were consistently detected in virion preparations via immunoblot analysis .
Table: Components of LIYV Virions
Product Specs
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Target Background
KEGG: vg:991076
