Recombinant Leuconostoc citreum GTPase Era (era)

Shipped with Ice Packs

In Stock

Cat. No.

BT35904

Source

Yeast

Synonyms

era; LCK_00713; GTPase Era

Purity

>85% (SDS-PAGE)

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Cat. No.

BT35904

Source

E.coli

Synonyms

era; LCK_00713; GTPase Era

Purity

>85% (SDS-PAGE)

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Cat. No.

BT35904

Source

E.coli

Synonyms

era; LCK_00713; GTPase Era

Purity

>85% (SDS-PAGE)

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Cat. No.

BT35904

Source

Baculovirus

Synonyms

era; LCK_00713; GTPase Era

Purity

>85% (SDS-PAGE)

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Cat. No.

BT35904

Source

Mammalian cell

Synonyms

era; LCK_00713; GTPase Era

Purity

>85% (SDS-PAGE)

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Description

Recombinant Production in L. citreum

L. citreum has been engineered for recombinant protein expression using bicistronic systems (BCD), which enhance translational efficiency . While Era-specific protocols are not documented, analogous workflows would involve:

Vector Design:
- Cloning the era gene under a constitutive promoter (e.g., cp-derived promoters ).
- Optimizing Shine-Dalgarno sequences (e.g., engineered SD2 in BCD systems) to boost expression .
Expression Outcomes:
- Expected yields: ~10–20 mg/L based on similar proteins (e.g., α-amylase) .
- Challenges: Era’s GTPase activity may require chaperones or co-expression with ribosomal components for stability.

Functional Insights from Homologs

Role in Ribosome Assembly:
Era ensures proper 16S rRNA folding by coordinating GTP hydrolysis with RNA binding, a mechanism conserved from bacteria to humans . Mutations in the GTPase domain (e.g., T99I in E. coli) disrupt ribosome biogenesis, leading to growth defects .
Regulatory Interactions:
Era’s activity is modulated by nucleotides (GDP/GTP) and ribosomal proteins (e.g., S18) . In L. citreum, analogous partnerships likely exist but remain uncharacterized.

Research Gaps and Future Directions

Unresolved Questions:
- Does L. citreum Era require unique cofactors?
- How does its KH domain recognize rRNA in this species?
Applications:
- Biotechnology: Engineered Era variants could optimize ribosome assembly in synthetic biology platforms.
- Disease Models: Studying Era homologs (e.g., ERAL1 in human mitochondria) informs therapies for ribosomopathies like Perrault syndrome .

Product Specs

Form

Lyophilized powder. We will preferentially ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will fulfill your request.

Lead Time

Delivery time varies based on purchasing method and location. Consult your local distributors for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance; additional fees apply.

Notes

Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.

Shelf Life

Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form is stable for 6 months at -20°C/-80°C, while the lyophilized form is stable for 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

The tag type will be determined during the manufacturing process. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

era; LCK_00713; GTPase Era

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-303

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Leuconostoc citreum (strain KM20)

Target Names

era

Target Protein Sequence

MAESTFKSGF VAIIGRPNVG KSTLLNRIVG EKIAIMSDKA QTTRNKIQGI YTTDNAQVVF IDTPGVHKPQ NSLGNFMVKS AFSALHEADA IWFVVDASMP RGRGDDFIIS RLNEVTETPI YLLINKVDLI AREELLAIIE SYQVDAPTWT EVFPISATEG DNVPELLDNV VSHLDEGPQY FDADQLTDHP ERFVIGELIR EKVLQLTRQE VPHSVAVVID KIAREDEEKI HIQASIIVER PTQKNIIIGK QGTMIKNIGT RARKDIERLM GDKVFLETWV KVEPRWRDRP QALQTLGYNE ENY

Uniprot No.

B1MYE3

Target Background

Function

An essential GTPase that binds GDP and GTP, with rapid nucleotide exchange. Involved in 16S rRNA processing, 30S ribosomal subunit biogenesis, and possibly cell cycle regulation and energy metabolism.

Database Links

KEGG: lci:LCK_00713

STRING: 349519.LCK_00713

Protein Families

TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, Era GTPase family

Subcellular Location

Cytoplasm. Cell membrane; Peripheral membrane protein.

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Recombinant Leuconostoc citreum GTPase Era (era)

Description

Recombinant Production in L. citreum

Functional Insights from Homologs

Research Gaps and Future Directions

Product Specs

Target Background

Quick Inquiry

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Service