Recombinant Leuconostoc citreum tRNA pseudouridine synthase A (truA)

Shipped with Ice Packs
In Stock

Product Specs

Form
Lyophilized powder. Note: We will preferentially ship the format we have in stock. If you have special format requirements, please specify them when ordering, and we will fulfill your request.
Lead Time
Delivery time varies depending on the purchasing method and location. Please consult your local distributors for specific delivery times. Note: All proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance; additional fees will apply.
Notes
Avoid repeated freezing and thawing. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect the contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on several factors, including storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you require a specific tag type, please inform us, and we will prioritize developing it.
Synonyms
truA; LCK_01564; tRNA pseudouridine synthase A; EC 5.4.99.12; tRNA pseudouridine(38-40) synthase; tRNA pseudouridylate synthase I; tRNA-uridine isomerase I
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-260
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Leuconostoc citreum (strain KM20)
Target Names
truA
Target Protein Sequence
MTRYKAIVAY DGSNFFGFQV QTKSGVEVQR TVQGELNKAV NQMAKQPVTP IKVVGASRTD TGVHAFGQVV HFDLPFDINP VGVSKGLNTL LPRDILIKAV TKVSDDFHAR FSTHAKRYFY RVSTTAFTDP FKRHYTGHFH WHLDTSRIQE ALPDLIGEHD FASFAASGNQ TASTVRTITS AQLIEKPDEQ ELVFVFEGNA FLYNQIRIMV GVLLEIGNGR REIHDIQRLI AVKDREQARF TAPASGLYLD EIDYGENPAN
Uniprot No.

Target Background

Function
Forms pseudouridine at positions 38, 39, and 40 in the anticodon stem and loop of transfer RNAs.
Database Links
Protein Families
TRNA pseudouridine synthase TruA family

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