Recombinant Listeria monocytogenes serotype 4b 5-deoxy-glucuronate isomerase (iolB)
Description
Overview of Listeria monocytogenes serotype 4b
Listeria monocytogenes is a Gram-positive, facultative intracellular bacterium responsible for listeriosis in humans and animals . Serotype 4b is notably associated with a high percentage of fatal, food-borne infections . L. monocytogenes serotype 4b strains belonging to lineages I and III possess distinct molecular features .
Recombinant Listeria monocytogenes serotype 4b 5-deoxy-glucuronate isomerase (iolB)
Recombinant Listeria monocytogenes serotype 4b 5-deoxy-glucuronate isomerase (iolB) is a protein that can be produced in various expression systems, including yeast, E. coli, baculovirus, and mammalian cells . It is also known as 5-deoxy-D-glucuronate isomerase and IolB, and is associated with the KEGG (Kyoto Encyclopedia of Genes and Genomes) number lmc:Lm4b_00402 .
IspC Autolysin
IspC is a surface-associated autolysin of L. monocytogenes serotype 4b . Monoclonal antibodies (MAbs) can recognize linear epitopes located within the cell wall binding domain (CBD) of IspC . IspC exhibits N-acetylglucosaminidase activity and is involved in virulence .
IspC is highly conserved within the 4b serotype, and some anti-IspC MAbs do not react with other L. monocytogenes serotypes, suggesting IspC is exclusive to L. monocytogenes serotype 4b . The high specificity of certain anti-IspC MAbs makes them candidates for use in the detection and capture of this clinically important serotype .
Pathogenicity of Serotype 4b
L. monocytogenes serotype 4b strains replicate more in CD11b-positive monocytes/macrophages in the liver compared to other serotypes . This characteristic may contribute to the pathogenicity of serotype 4b strains, particularly in the dissemination of L. monocytogenes throughout the host body .
Monoclonal Antibodies (MAbs)
MAbs generated against formalin-killed L. monocytogenes serotype 4b whole cells often target the same surface protein, IspC, suggesting it is an immuno-dominant surface antigen . Some MAbs, such as M2774, M2775, M2780, M2790, and M2797, exhibit high specificity for L. monocytogenes serotype 4b, with minimal cross-reactivity to non-serotype 4b isolates, except for serotype 4ab isolates .
Product Specs
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Target Background
KEGG: lmf:LMOf2365_0396
