Recombinant Listeria monocytogenes serotype 4b Glutamate--tRNA ligase (gltX)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please consult your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, which can serve as a reference.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses to prevent repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
gltX; LMOf2365_0249; Glutamate--tRNA ligase; EC 6.1.1.17; Glutamyl-tRNA synthetase; GluRS
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-491
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Listeria monocytogenes serotype 4b (strain F2365)
Target Names
gltX
Target Protein Sequence
MSETKRVRVR YAPSPTGFLH IGNARTALFN YLFARHNDGD FIIRIEDTDA KRNIADGEES
QMTNLKWLGM DWDEGVDVPG KYGPYRQSER QSIYEPLIQQ LLDEGLAYKC YCTEEELDAE
REKQKANGEM PRYSGKCRHL TKEQQAEKEA QGFKPSIRFK VPANETITFN DMVKDDVSFE
SNGIGDFVIA KKDGIPTYNF AVAVDDHLME ISHVLRGDDH ISNTPKQILI YNAFGWEPPI
FGHMTLIVNE SRRKLSKRDG SIIQFIEQYR DLGYLPEALF NFIAMLGWSP EGEEEIFSKE
EFIKMFDPKR LSKSPALFDN VKLTWVNNQY VKKLPLNDVV ELSLPHLQKA GVVSAELDQA
ELDWVHKLVS LYHEQMSYGA EIVPLSEMFF ADAESITFDE EETAVLAEET VPTVISAFKK
ELEALEVLEA AEVKAAIKRV QKETGVKGKG LFMPIRIVTT GEMHGPELPL AIEVLGREKV
LNRLDTWLKN N
Uniprot No.
Target Background
Function
This enzyme catalyzes the two-step attachment of glutamate to tRNA(Glu): glutamate is initially activated by ATP to form Glu-AMP, followed by transfer to the tRNA(Glu) acceptor end.
Database Links
KEGG: lmf:LMOf2365_0249
Protein Families
Class-I aminoacyl-tRNA synthetase family, Glutamate--tRNA ligase type 1 subfamily
Subcellular Location
Cytoplasm.
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