Recombinant Listeria monocytogenes serotype 4b Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please consult your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a reference.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses to prevent repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
The tag type is finalized during production. If you require a specific tag, please inform us for prioritized development.
The tag type is finalized during production. If you require a specific tag, please inform us for prioritized development.
Synonyms
gatA; LMOf2365_1780; Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-483
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Listeria monocytogenes serotype 4b (strain F2365)
Target Names
gatA
Target Protein Sequence
MGLFDFSVKE LHDKLVKKEI SPFDLVSESF NRIESVEDKV GSFITLNKEA AFGVAEELGD
AGIDPNNMLS GLPIGIKDNI VTKNLRTTAA SKILENFDPI YDATVVSKLK NAQTINIGKL
NMDEFAMGSS TETSYFHKTH NPWDLSRVPG GSSGGSASAV AAGEVLFSLG SDTGGSIRQP
AAFCGVVGMK PTYGRVSRFG LIAFASSLDQ IGPITKNVED NAYLLEAISG LDANDSTSIN
QPVERFSDSL TGDIKGLRIG VPKEYLGEGV DPGVKQAVLD ALKTLEKLGA TWDEVSLPHS
EYGVASYYIL ASSEASSNLS RFDGVRYGYR SPNATTLEEL YTKTRSEGFG DEVKRRIMLG
TYALSSGYYD AYYKKAQQAR TLIKQDFVNV FENYDVIIGP SSPTTAFKID GMINDPITMY
SNDILTVPIN LAGVPAISVP CGFSDGLPVG LQIIGNYFEE SLLYKVAHAF EQETTFHKEK
PNL
Uniprot No.
Target Background
Function
This protein facilitates the accurate formation of Gln-tRNA(Gln) by transamidating misacylated Glu-tRNA(Gln). This is crucial in organisms lacking glutaminyl-tRNA synthetase. The reaction utilizes glutamine and ATP to activate gamma-phospho-Glu-tRNA(Gln).
Database Links
KEGG: lmf:LMOf2365_1780
Protein Families
Amidase family, GatA subfamily
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