Recombinant Litoria peronii Peroniin-1.3

Shipped with Ice Packs
In Stock
Cat. No.
BT56233
Source
Yeast
Synonyms
Peroniin-1.3
Purity
>85% (SDS-PAGE)
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Cat. No.
BT56233
Source
E.coli
Synonyms
Peroniin-1.3
Purity
>85% (SDS-PAGE)
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Cat. No.
BT56233
Source
E.coli
Synonyms
Peroniin-1.3
Purity
>85% (SDS-PAGE)
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Cat. No.
BT56233
Source
Baculovirus
Synonyms
Peroniin-1.3
Purity
>85% (SDS-PAGE)
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Cat. No.
BT56233
Source
Mammalian cell
Synonyms
Peroniin-1.3
Purity
>85% (SDS-PAGE)
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Description

Recombinant Production Methods

Peroniin-1.3a is commercially produced in two recombinant forms using distinct expression systems:

Escherichia coli (E. coli)-Derived Peroniin-1.3a

  • Product Code: CSB-EP309624LHO

  • Purity: >85% (SDS-PAGE verified)

  • Reconstitution: Solubilized in deionized water (0.1–1.0 mg/mL) with 5–50% glycerol for stability .

Yeast-Derived Peroniin-1.3a

  • Product Code: CSB-YP309624LHO

  • Purity: >85% (SDS-PAGE verified)

  • Storage: -20°C (short-term) or -80°C (long-term) .

Both variants retain the native sequence but differ in expression tags, which are determined during manufacturing .

Role in Amphibian Defense

Peroniin-1.3a is expressed in dorsal skin glands and functions as a defensive peptide against pathogens . While its specific antimicrobial activity remains uncharacterized, transcriptomic studies of L. peronii relatives (e.g., Rhinella schneideri) highlight its classification among AMPs like brevinins and buforins .

Transcriptomic Prevalence

In R. schneideri skin gland analyses, peroniin-family peptides showed moderate expression levels:

AMP FamilyTranscripts Per Million (TPM)
Buforin766.95
Peroniin129.53–841.55
Brevinin122.99–175.33

Peroniin-1.3a specifically registered a TPM of 129.53, indicating constitutive expression in cutaneous secretions .

Handling and Stability

  • Reconstitution: Avoid repeated freeze-thaw cycles; working aliquots stored at 4°C are stable for ≤1 week .

  • Shelf Life:

    • Liquid: 6 months at -80°C

    • Lyophilized: 12 months at -80°C .

Research Gaps and Future Directions

  • Activity Data: No minimum inhibitory concentration (MIC) or cytotoxicity data are available .

  • Structural Studies: Predicted tertiary structures and binding targets are uncharacterized .

  • Comparative Analyses: Functional differences between native and recombinant variants require validation.

Product Specs

Form
Lyophilized powder. We will ship the in-stock format preferentially. If you have specific format requirements, please note them when ordering.
Lead Time
Delivery times vary by purchase method and location. Consult your local distributor for specifics. All proteins ship with standard blue ice packs. Request dry ice in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Working aliquots are stable at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us and we will prioritize its development.
Synonyms
Peroniin-1.3
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-7
Protein Length
Cytoplasmic domain
Purity
>85% (SDS-PAGE)
Species
Litoria peronii (Emerald spotted tree frog) (Hyla peronii)
Target Protein Sequence
QPWLPFV
Uniprot No.
P86492

Target Background

Function
Induces contraction of intestinal smooth muscle in isolated guinea pig ileum.
Protein Families
Frog skin active peptide (FSAP) family, Peroniin subfamily
Subcellular Location
Secreted.
Tissue Specificity
Expressed by the skin dorsal glands.

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