Recombinant Loxosceles arizonica Sphingomyelin phosphodiesterase D LarSicTox-alphaIB2a
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we will prioritize shipping the format currently in stock, please specify any format requirements in your order notes for customized preparation.
Note: While we will prioritize shipping the format currently in stock, please specify any format requirements in your order notes for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please consult your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs unless otherwise requested. Dry ice shipping requires prior arrangement and incurs additional charges.
Note: All proteins are shipped with standard blue ice packs unless otherwise requested. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a guideline.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
Dermonecrotic toxin LarSicTox-alphaIB2a; EC 4.6.1.-; Phospholipase D; PLD; Sphingomyelin phosphodiesterase D 1; SMD 1; SMase D 1; Sphingomyelinase D 1
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
42-320
Protein Length
Full Length of Mature Protein
Purity
>85% (SDS-PAGE)
Species
Loxosceles arizonica (Arizona brown spider)
Target Protein Sequence
ANKRPIWIM GHMVNANYQI
DEFVNLGANS IETDVSFDSS ANPEYTYHGV PCDCRGWCKK WEYFNNFLKA LRKATTPGDS
KYHEKLVLVV FDLKTGSLYD NQAYDAGKKL AKNLLQHYWN NGNNGGRAYI VLSIPNLAHY
KLITGFKETL KTEGHPELME KVGYDFSGND NIDQVANAYK KAGVTGHVWQ SDGITNCLLR
GLDRVRKAVA NRDSSNGYIN KVYYWTVDKR QSTKNALDAG VDGIMPNYPD VIADVPNESA
YKAKFRIASY DDNPWETFKN
Uniprot No.
Target Background
Function
Dermonecrotic toxins cleave the phosphodiester bond between the phosphate group and headgroup of specific phospholipids (sphingolipids and lysolipids), generating an alcohol (often choline) and a cyclic phosphate. This toxin targets sphingomyelin (SM) and may also act on ceramide phosphoethanolamine (CPE), lysophosphatidylcholine (LPC), and lysophosphatidylethanolamine (LPE), but not lysophosphatidylserine (LPS) or lysophosphatidylglycerol (LPG). Its mechanism involves transphosphatidylation, exclusively releasing cyclic phosphate products. The resultant effects include dermonecrosis, hemolysis, increased vascular permeability, edema, inflammation, and platelet aggregation.
Protein Families
Arthropod phospholipase D family, Class II subfamily
Subcellular Location
Secreted.
Tissue Specificity
Expressed by the venom gland.
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