Recombinant Porphyromonas gingivalis Lys-gingipain HG66 (kgp), partial

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Cat. No.
BT33398
Source
Yeast
Synonyms
kgpLys-gingipain HG66; EC 3.4.22.47) [Cleaved into: Lys-gingipain catalytic subunit; 39 kDa adhesin; 15 kDa adhesin; 44 kDa adhesin]
Purity
>85% (SDS-PAGE)
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Cat. No.
BT33398
Source
E.coli
Synonyms
kgpLys-gingipain HG66; EC 3.4.22.47) [Cleaved into: Lys-gingipain catalytic subunit; 39 kDa adhesin; 15 kDa adhesin; 44 kDa adhesin]
Purity
>85% (SDS-PAGE)
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Cat. No.
BT33398
Source
Baculovirus
Synonyms
kgpLys-gingipain HG66; EC 3.4.22.47) [Cleaved into: Lys-gingipain catalytic subunit; 39 kDa adhesin; 15 kDa adhesin; 44 kDa adhesin]
Purity
>85% (SDS-PAGE)
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Cat. No.
BT33398
Source
Mammalian cell
Synonyms
kgpLys-gingipain HG66; EC 3.4.22.47) [Cleaved into: Lys-gingipain catalytic subunit; 39 kDa adhesin; 15 kDa adhesin; 44 kDa adhesin]
Purity
>85% (SDS-PAGE)
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Description

Functional Domains and Biotype Specificity

Kgp HG66 exhibits distinct biotype-specific traits compared to other P. gingivalis strains:

BiotypeKgp FeaturesFunctional Implications
HG66Soluble secretion; truncated HA region; lacks C-terminal anchor peptide .Enhanced extracellular proteolytic activity; altered immune evasion mechanisms .
381Membrane-bound; full HA region with cleaved adhesin domains .Stronger adherence to host cells; efficient heme acquisition .
W83Membrane-bound; duplicated hemagglutination motifs in Kgp44 domain .Increased erythrocyte lysis and biofilm formation .

The HG66 biotype’s unique processing of Kgp enables it to evade host immune responses by degrading complement components (e.g., C3, C5) and neutrophil receptors . Its soluble nature facilitates systemic dissemination in periodontal tissues .

Expression and Purification

  • Host System: Expressed in E. coli with >90% purity via affinity chromatography .

  • Storage: Tris-based buffer with 50% glycerol; stable for 6 months at -80°C .

Functional Motifs

MotifSequenceRole
Catalytic SiteCys244-His211Hydrolysis of lysine-terminal peptides .
HaemagglutinationPVQNLTErythrocyte binding and haemolysis .
Immunogenic EpitopeG907-T931Target for neutralizing antibodies .

Research Implications

  • Therapeutic Development: Structural insights into the S1 lysine-binding pocket (resolution: 1.9 Å) enable rational design of inhibitors like FFRCMK analogs .

  • Pathogenicity Studies: The HG66 Kgp’s soluble form disrupts neutrophil chemotaxis by cleaving C5a receptors and FMLP receptors .

  • Diagnostics: Antibodies targeting the immunogenic epitope (G907-T931) show potential for periodontal disease biomarkers .

Product Specs

Form
Lyophilized powder. We will ship the available format, but you can specify your preference when ordering.
Lead Time
Delivery times vary by location and purchase method. Contact your local distributor for details. Proteins are shipped with blue ice packs. Request dry ice in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Default glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please let us know and we will prioritize its development.
Synonyms
kgpLys-gingipain HG66; EC 3.4.22.47) [Cleaved into: Lys-gingipain catalytic subunit; 39 kDa adhesin; 15 kDa adhesin; 44 kDa adhesin]
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Porphyromonas gingivalis
Target Names
kgp
Uniprot No.
P72197

Target Background

Function
Lys-gingipain (Kgp) is a cysteine proteinase preferring substrates with Lys at P1. It degrades hemoglobin, serum albumin, casein, collagen, and IgA/IgG. It impairs polymorphonuclear leukocyte function and may contribute to periodontal disease by acting as a virulence factor and mediating bacterial coaggregation.
Protein Families
Peptidase C25 family
Subcellular Location
[Lys-gingipain catalytic subunit]: Secreted.

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