Recombinant Macaca fascicularis Non-secretory ribonuclease (RNASE2)
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Description
Table 1: Impact of Key Mutations on RNase Activity
| Mutation | RNase Activity (nmol RNA/s/nmol RNase) | Fold Change vs. Wild-Type | Source |
|---|---|---|---|
| Wild-Type EDN | 4.2 | — | |
| Arg-132 → Thr (GZ-C) | 0.35 | 12× ↓ | |
| Ser-64 → Arg (GZ-F) | 0.057 | 74× ↓ | |
| Arg-64/Thr-132 → Ser/Arg | 13.0 | 15× ↑ |
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The Thr-132→Arg substitution stabilizes substrate binding via electrostatic interactions with RNA phosphate groups .
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Synergistic effects of Ser-64 and Arg-132 enhance catalytic efficiency by 13-fold in ancestral reconstructions .
Antiviral Mechanisms
Target Pathogen: Respiratory Syncytial Virus (RSV)
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Binds extracellular RSV virions via N-terminal residues (34–38), promoting RNA degradation .
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Antiviral activity is saturable and specific, unaffected by structurally unrelated RNases like RNase k6 .
Table 2: Antiviral Efficacy Against RSV
| Protein | Infectious Units (% of Control) | Significance (p-value) | Source |
|---|---|---|---|
| Control Buffer | 100% | — | |
| Human EDN | 47% | <0.001 | |
| Owl Monkey EDN | 118% | NS | |
| Ancestral EDN (Node A) | 78% | <0.001 |
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Evolutionary acquisition of antiviral activity occurred post-divergence of Old World (OW) and New World (NW) monkeys .
Evolutionary Insights
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Gene Duplication: EDN diverged from ECP (~30 million years ago), gaining enhanced RNase activity through complementary substitutions .
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Functional Divergence:
Applications and Future Directions
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Therapeutic Potential:
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Research Tools:
Product Specs
Form
Lyophilized powder. We will ship the in-stock format preferentially. If you have special format requirements, please note them when ordering, and we will accommodate your request.
Lead Time
Delivery times vary depending on the purchase method and location. Please consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance; additional fees apply.
Notes
Avoid repeated freezing and thawing. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on several factors including storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C. The lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you require a specific tag type, please inform us, and we will prioritize developing it.
Synonyms
RNASE2; EDN; RNS2; Non-secretory ribonuclease; EC 4.6.1.18; Eosinophil-derived neurotoxin; RNase UpI-2; Ribonuclease 2; RNase 2; Ribonuclease US
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
28-160
Protein Length
Full Length of Mature Protein
Purity
>85% (SDS-PAGE)
Species
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Target Names
RNASE2
Target Protein Sequence
KPG QFTWAQWFEI QHINMTSGQC TNAMQVINNY
QRRCKNQNTF LLTTFADVVH VCGNPSMPCP SNTSLNNCHH SGVQVPLIHC NLTTPSRRIS
NCRYTQTTAN KYYIVACNNS DPVRDPPQYP VVPVHLDRII
Uniprot No.
Target Background
Function
This is a non-secretory, pyrimidine-specific ribonuclease with a slight preference for uracil (U). It functions as a cytotoxin and helminthotoxin and exhibits a wide range of biological activities.
Protein Families
Pancreatic ribonuclease family
Subcellular Location
Lysosome. Cytoplasmic granule. Note=Matrix of eosinophil's large specific granule.
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