Recombinant Marchantia polymorpha ATP synthase subunit a (ATP6)
Description
Functional Role in ATP Synthase
ATP6 is a core component of the mitochondrial F₀ domain, facilitating proton translocation across the inner membrane to drive ATP synthesis. Key functional insights:
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Proton channel: Contains conserved residues (e.g., arginine in helix 5) critical for electrostatic interactions with subunit c (c-ring) during proton transport .
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Assembly: Requires chaperones like Atp10 and Atp23 for stability and integration into the F₀ complex .
3.1. Biochemical Studies
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SDS-PAGE analysis: Used to verify purity and molecular weight .
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ELISA: Commercial kits (e.g., MyBioSource) enable quantitative detection in mitochondrial extracts .
3.2. Comparative Mitochondrial Research
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RNA editing defects: In Sorghum bicolor, reduced atp6 RNA editing correlates with cytoplasmic male sterility (CMS), highlighting its role in pollen development .
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Chimeric ORFs: In Mimulus guttatus, rearrangements near atp6 are linked to CMS, suggesting evolutionary conservation of its regulatory regions .
Production and Handling Protocols
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Reconstitution: Dissolve in sterile water (0.1–1.0 mg/mL) with 5–50% glycerol for long-term storage .
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Stability: Working aliquots stored at 4°C retain activity for ≤1 week .
Evolutionary and Medical Relevance
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Conservation: ATP6 homologs in bovines (UniProt P00847) and yeast share structural motifs, underscoring its essential role across eukaryotes .
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Human diseases: Mutations in human MT-ATP6 cause mitochondrial encephalopathies, making Marchantia’s ATP6 a model for studying pathogenic variants .
Key Research Findings
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Cell-type-specific editing: In CMS Sorghum, atp6 RNA editing drops to 20% in sterile anthers vs. 100% in fertile lines, restored in hybrids (F1/F2) .
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Assembly-coupled translation: Yeast studies show ATP6 synthesis increases when assembly intermediates accumulate, suggesting feedback regulation .
Comparative Analysis of ATP6 Across Species
| Species | Marchantia polymorpha | Bos taurus | Saccharomyces cerevisiae |
|---|---|---|---|
| Gene | ATP6 (mtDNA) | MT-ATP6 (mtDNA) | ATP6 (mtDNA) |
| Protein Length | 252 aa | 226 aa | 248 aa |
| Key Function | Proton channel | Proton channel | Proton channel |
| Disease Association | N/A | Leigh syndrome | N/A |
Future Directions
Product Specs
Note: We will prioritize shipping the format we have in stock. However, if you have any specific format requirements, please indicate them when placing your order. We will fulfill your request based on availability.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance. Additional fees may apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
