Recombinant Mesoplasma florum tRNA pseudouridine synthase B (truB)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is requested in advance. Additional fees apply for dry ice shipping.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50%, which can serve as a guideline.
Shelf Life
Shelf life depends on various factors, including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
truB; Mfl282; tRNA pseudouridine synthase B; EC 5.4.99.25; tRNA pseudouridine(55) synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-286
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum)
Target Names
truB
Target Protein Sequence
MNKSGIIILN KPKGLTTNHL IQKLKRKLNV KKIGHAGTLD PLATGVVICL INNGTKLSDY
FLNENKAYEV TMKLFQATDT YDSDGSIIEE QEPFKIEQEQ VEKVIAKFNG LAYEQEPPMY
SAIKIDGKKL YEYARENQVV KVNKRLIKIN SLSLDKYENN EISMTVYCSK GTYIRSLIVD
IAKELNTIAH VTSLNRIESG NFIIKNSVNI ENCEESNLIK MFDAIKMADY EIVELDDTLN
VEHGKKIEVI AEKNIVFISN KSKELIACYE RENGNVFKCK RGGLNI
Uniprot No.
Target Background
Function
This protein is responsible for the synthesis of pseudouridine from uracil-55 in the ψGC loop of transfer RNAs.
Database Links
KEGG: mfl:Mfl282
STRING: 265311.Mfl282
Protein Families
Pseudouridine synthase TruB family, Type 1 subfamily
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