Recombinant Metarhizium robertsii Signal peptidase complex catalytic subunit SEC11 (SEC11)
Description
Introduction to Recombinant Metarhizium robertsii Signal Peptidase Complex Catalytic Subunit SEC11 (SEC11)
The Recombinant Metarhizium robertsii Signal peptidase complex catalytic subunit SEC11 (SEC11) is a recombinant protein derived from the fungus Metarhizium robertsii. This protein is part of the signal peptidase complex, which plays a crucial role in the processing of secreted proteins by removing the signal peptide, allowing these proteins to be correctly targeted and secreted outside the cell. The SEC11 protein is specifically involved in this process, ensuring that proteins destined for secretion are properly processed.
Characteristics of Recombinant SEC11
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Expression System: The recombinant SEC11 protein is expressed in Escherichia coli (E. coli), a common host organism for recombinant protein production due to its well-understood genetics and ease of manipulation .
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Protein Structure: The SEC11 protein consists of 172 amino acids and is fused with an N-terminal His tag, which facilitates purification using affinity chromatography .
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Function: As part of the signal peptidase complex, SEC11 is involved in the cleavage of signal peptides from newly synthesized proteins, enabling them to be secreted or embedded in membranes correctly.
Table: Characteristics of Recombinant SEC11
| Characteristic | Description |
|---|---|
| Expression System | Escherichia coli |
| Protein Length | 172 amino acids |
| Tag | N-terminal His tag |
| Function | Signal peptide cleavage |
Potential Applications in Biotechnology
The recombinant SEC11 protein could have applications in biotechnology, particularly in improving the secretion efficiency of proteins in fungal systems. This could enhance the production of enzymes or other proteins of interest for industrial or agricultural use.
References
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Liao X, Fang W, Lin L, Lu H-L, Leger RJS (2013). Metarhizium robertsii Produces an Extracellular Invertase (MrINV) That Plays a Pivotal Role in Rhizospheric Interactions and Root Colonization. PLoS ONE 8(10): e78118.
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Creative BioMart. Recombinant Full Length Metarhizium Robertsii Signal Peptidase Complex Catalytic Subunit Sec11(Sec11) Protein, His-Tagged.
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Frontiers in Fungal Biology. Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets of Metarhizium robertsii.
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized fulfillment.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize development accordingly.
Target Background
The recombinant Metarhizium robertsii Signal Peptidase Complex catalytic subunit SEC11 (SEC11) is a catalytic component of the signal peptidase complex (SPC). It catalyzes the cleavage of N-terminal signal sequences from proteins destined for the endoplasmic reticulum. This signal peptide cleavage occurs during translocation (co-translationally or post-translationally) through the translocon pore into the endoplasmic reticulum.
KEGG: maj:MAA_08708
