Recombinant Methanococcus maripaludis Diphthine synthase (dphB)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes. We will fulfill requests whenever possible.
Lead Time
Delivery times vary depending on the purchase method and location. Please consult your local distributor for precise delivery estimates.
Note: All proteins are shipped on standard blue ice packs unless otherwise requested. Dry ice shipping requires advance notice and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and may serve as a useful reference.
Shelf Life
Shelf life depends on various factors, including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is crucial for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If a specific tag type is required, please inform us; we will prioritize its inclusion in the production process.
Synonyms
dphB; dph5; MMP0588Diphthine synthase; EC 2.1.1.98; Diphthamide biosynthesis methyltransferase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-255
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Methanococcus maripaludis (strain S2 / LL)
Target Names
dphB
Target Protein Sequence
MLVMAGLGLY DERDVTLKTL DFAKKVDKIY AEFYTAILTG TTMEKIEGTL QKPITVLDRE
KVEYETNKLI DEAKDKDIMF LTAGDPMVAT THVDIAVEAR KKGIEVVIIN APSIYSAIGI
TGLQLYKFGK TTSIVFPEPN YFPETPYDVI KDNLKLGYHT LCLLDIQADK ERFMTANEGL
DALLKIEEKR KENVISEETE VAVVARAGSI NPGLYYGKIK DLLNYDFKSP LHCVIIPGKL
HFMEEDALKY LFENI
Uniprot No.
Target Background
Function
S-adenosyl-L-methionine-dependent methyltransferase catalyzing the trimethylation of the amino group of a modified target histidine residue in translation elongation factor 2 (EF-2), forming the intermediate diphthine. This three-step methylation process constitutes the second step in diphthamide biosynthesis.
Database Links
KEGG: mmp:MMP0588
STRING: 267377.MMP0588
Protein Families
Diphthine synthase family
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
