Recombinant Methanopyrus kandleri Protein translocase subunit SecF (secF)
Description
Role in Protein Translocation
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Primary Function: Facilitates efficient translocation of unfolded proteins across the cytoplasmic membrane by stabilizing the SecYE translocon .
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Proton Motive Force (PMF) Interaction: Enhances the rate of translocation via interactions with the PMF, though its exact mechanism in archaea remains under investigation .
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Non-Essential but Critical: While not indispensable for translocation per se, SecF significantly improves the efficiency of the process .
Protein Translocation Studies
Recombinant SecF is used to investigate:
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Mechanisms of Cotranslational vs. Posttranslational Translocation: While archaeal Sec systems lack SecA (absent in M. kandleri), SecF’s role in stabilizing the translocon during PMF-dependent export is a focus .
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Membrane Protein Biogenesis: Studies on how SecF interacts with the SecYE complex to facilitate insertion of hydrophobic segments into the membrane .
ELISA and Immunological Assays
Commercial ELISA kits (e.g., CSB-CF844835MSI) utilize recombinant SecF for:
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Antibody Validation: Detecting anti-SecF antibodies in serum or purified samples .
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Quantitative Protein Analysis: Measuring SecF concentrations in M. kandleri lysates or recombinant preparations .
Evolutionary Conservation
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Cross-Domain Homology: SecF’s role in enhancing translocation is conserved in bacteria and archaea, despite differences in accessory components like SecA .
Table 1: Amino Acid Sequence Highlights
Table 2: Recombinant Production Parameters
Product Specs
Note: While we prioritize shipping the format currently in stock, we are happy to accommodate specific requests. If you have a preference, please indicate it in your order notes and we will fulfill your requirement.
Note: All protein shipments are standardly packaged with blue ice packs. If you require dry ice, please contact us in advance as an additional fee will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: mka:MK1008
STRING: 190192.MK1008
