Recombinant Methylobacillus flagellatus Disulfide bond formation protein B (dsbB)
Description
Protein Overview
DsbB (UniProt ID: Q1H058) belongs to the disulfide oxidoreductase family and operates in the bacterial periplasm. In M. flagellatus, it is encoded by the dsbB gene (locus tag: Mfla_1862) and expressed as a 166-amino-acid protein with a predicted molecular weight of ~18.5 kDa (calculated from sequence data) .
Functional Role in Oxidative Folding
DsbB acts as a redox transducer, transferring electrons from the periplasmic oxidoreductase DsbA to membrane-bound quinones (e.g., ubiquinone). This cycle ensures DsbA remains oxidized, enabling it to catalyze disulfide bond formation in substrate proteins .
Mechanistic Insights:
Recombinant Expression and Applications
The recombinant form (rDsbB) is produced in E. coli systems for biochemical studies. Key production details include:
Production Parameters :
| Parameter | Detail |
|---|---|
| Expression system | E. coli (optimized codon usage) |
| Tag | Undisclosed (determined during production) |
| Storage | Tris buffer, 50% glycerol; stable at -20°C or -80°C |
| Purity | >90% (SDS-PAGE verified) |
Sequence highlights:
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Amino acids 1–166 (full-length).
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Conserved motifs:
CXXC(residues 41–44, 104–107) and transmembrane domains (residues 15–37, 50–72, etc.) .
Research Findings in Methylotrophy
DsbB’s role in M. flagellatus is indirectly linked to its methylotrophic metabolism:
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Metabolic redundancy: M. flagellatus employs parallel pathways for formaldehyde oxidation (cyclic vs. linear). DsbB likely stabilizes enzymes like 6-phosphogluconate dehydrogenase (GndA/B), which are critical for the cyclic pathway .
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Mutant phenotypes:
Comparative Analysis with Homologs
Future Directions
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it in your order notes. We will accommodate your request whenever possible.
Note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipment, please contact us in advance as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: mfa:Mfla_1862
STRING: 265072.Mfla_1862
