Recombinant Methylobacterium extorquens Glycerol-3-phosphate acyltransferase (plsY)
Description
Introduction to Recombinant Methylobacterium extorquens Glycerol-3-Phosphate Acyltransferase (plsY)
Recombinant Methylobacterium extorquens glycerol-3-phosphate acyltransferase (PlsY) is a bacterial enzyme engineered for biochemical and biotechnological applications. It catalyzes the transfer of acyl groups from acyl-phosphate to glycerol-3-phosphate, a critical step in bacterial phospholipid biosynthesis. The enzyme is produced via recombinant expression in E. coli, with a His-tag for purification and stability. Its full-length sequence (1–202 amino acids) corresponds to the UniProt identifier A9W739, and it shares conserved structural motifs with homologs in other bacteria .
Biological Role in Phospholipid Biosynthesis
PlsY operates in the PlsX-Y pathway, a widespread bacterial mechanism for initiating phospholipid synthesis:
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PlsX converts acyl-acyl carrier protein (acyl-ACP) to acyl-phosphate.
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PlsY transfers the acyl group from acyl-phosphate to glycerol-3-phosphate, forming lysophosphatidic acid (LPA).
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LPA acyltransferase adds a second acyl group to produce phosphatidic acid, a precursor for membrane phospholipids .
This pathway is essential for maintaining membrane integrity, particularly in methylotrophic bacteria like M. extorquens, which face oxidative stress during methanol metabolism .
Production and Purification of Recombinant PlsY
Recombinant PlsY is produced in E. coli with a His-tag for affinity chromatography. Key specifications include:
| Parameter | Value |
|---|---|
| Source | Methylobacterium extorquens AM1 |
| Expression Host | E. coli |
| Tag | N-terminal His-tag |
| Protein Length | Full-length (1–202 amino acids) |
| Purity | >90% (SDS-PAGE) |
| Storage Buffer | Tris/PBS-based buffer with 6% trehalose (pH 8.0) |
| Reconstitution | Lyophilized powder dissolved in sterile water (0.1–1.0 mg/mL) |
Notes: Avoid repeated freeze-thaw cycles. Aliquots with 50% glycerol are recommended for long-term storage at −20°C/−80°C .
Functional Studies
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Mutagenesis:
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Inhibition: Noncompetitive inhibition by palmitoyl-CoA indicates allosteric regulation .
Applications in Biotechnological and Biochemical Studies
Recombinant PlsY is used to study:
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Lipid biosynthesis pathways in methylotrophic bacteria, particularly under methanol-induced stress .
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Membrane engineering to enhance stress resistance or modify lipid composition for biofuel production.
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Enzyme kinetics and inhibitor screening, leveraging its conserved motifs and inhibition profiles .
Product Specs
Please note: We prioritize shipping the format currently in stock. If you have specific requirements for the format, please indicate them in your order notes, and we will fulfill your request to the best of our ability.
Please note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance. Additional fees may apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: mex:Mext_3314
STRING: 419610.Mext_3314
