Recombinant Methylobacterium populi Membrane protein insertase YidC (yidC)
Description
Introduction to YidC Insertase
YidC is a universally conserved membrane protein insertase critical for the co-translational integration, folding, and assembly of membrane proteins across bacteria, mitochondria, and chloroplasts . In bacteria, YidC operates independently or alongside the Sec translocon, facilitating substrate insertion at the membrane-lipid interface through a conserved hydrophilic cavity . Methylobacterium populi, a Gram-negative bacterium known for xenobiotic biodegradation and plant growth promotion , encodes its own YidC homolog, which has been recombinantly produced for structural and functional studies .
Mechanistic Role in Membrane Protein Biogenesis
YidC mediates substrate insertion via a hydrophobic slide formed by transmembrane segments (e.g., TM3 and TM5) . Structural models reveal a hydrophilic cavity accessible from the cytoplasm and lipid phase, enabling substrate interaction at the membrane interface . Mutations in these regions disrupt substrate insertion efficiency .
Substrate Specificity and Cooperativity
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Sec-Independent Insertion: YidC autonomously integrates single-spanning proteins (e.g., Pf3 coat protein) .
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Sec-YEG Collaboration: Assists in the biogenesis of multi-pass membrane proteins (e.g., F0c subunit of ATP synthase) .
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Partner Proteins: Interaction with YibN enhances the insertion of YidC-dependent substrates like SecG and F0c .
Biophysical Studies
Recombinant YidC enables in vitro assays to study:
Comparative Analysis of YidC Homologs
Challenges and Future Directions
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Structural Resolution: High-resolution cryo-EM or crystallography studies are needed to elucidate substrate-binding mechanisms.
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Ecological Relevance: Link YidC’s role in M. populi to its bioremediation and plant growth-promoting traits .
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Biotechnological Applications: Engineer YidC for synthetic membrane protein production or industrial biocatalysis .
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific requirements for the format, please specify them in your order. We will prepare the product according to your needs.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance, as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: mpo:Mpop_2308
STRING: 441620.Mpop_2308
