Recombinant Methylococcus capsulatus Prolipoprotein diacylglyceryl transferase (lgt)
Description
Enzymatic Function and Biological Role
Lgt performs the first step in lipoprotein maturation, enabling downstream modifications by signal peptidase II (Lsp) and apolipoprotein N-acyltransferase (Lnt). This process is essential for bacterial viability, as demonstrated by growth defects in lgt depletion strains . Key functional characteristics include:
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Substrate specificity: Targets lipobox motifs (e.g., -Leu-Ala/Ser-Gly-Ala-Cys-) in prolipoproteins .
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Membrane topology: Seven transmembrane helices with periplasmic N-terminus and cytoplasmic C-terminus, positioning the active site for lipid transfer .
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Catalytic residues: Conserved residues (e.g., Y26, N146, G154 in E. coli) are critical for enzymatic activity .
Relevance in Methylococcus capsulatus
While M. capsulatus Lgt has not been directly characterized, its genome encodes homologs of lipoprotein modification enzymes . Key contextual findings:
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Metabolic engineering: Recombinant M. capsulatus strains have been developed for industrial applications (e.g., succinic acid production), though these studies focus on central carbon metabolism rather than lipoprotein processing .
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Capsule biosynthesis: M. capsulatus produces a polysaccharide capsule, suggesting lipoprotein-dependent membrane anchoring mechanisms analogous to E. coli colanic acid systems .
Biotechnological Implications
Lgt’s role in lipoprotein biogenesis makes it a potential target for:
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Antibiotic development: Essentiality in pathogenic bacteria (e.g., Helicobacter pylori) highlights its therapeutic promise .
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Industrial strain optimization: Enhancing lipoprotein-dependent secretion systems in methanotrophs for biofuel or SCP production .
Research Gaps and Future Directions
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it in your order notes, and we will accommodate your request.
Note: All proteins are shipped with standard blue ice packs. If dry ice shipping is required, please communicate this in advance as additional fees will apply.
Generally, the shelf life for the liquid form is 6 months at -20°C/-80°C. Lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: mca:MCA2727
STRING: 243233.MCA2727
