Recombinant Microcebus griseorufus NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L)
Description
Functional Role in Mitochondrial Complex I
MT-ND4L is a core subunit of Complex I (NADH:ubiquinone oxidoreductase), which catalyzes:
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Electron Transfer: NADH → FMN → Fe-S clusters → ubiquinone (CoQ10) .
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Proton Pumping: Four protons translocated per NADH oxidized, driving ATP synthesis .
Key functional regions include:
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Transmembrane Helices: Critical for proton channel formation .
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Overlap with MT-ND4: In humans, a 7-nucleotide overlap between MT-ND4L and MT-ND4 genes ensures coordinated translation .
Research Applications
Recombinant MT-ND4L is utilized in:
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Disease Modeling:
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Structural Studies:
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Drug Screening: Targeting proton-pumping mechanisms for metabolic disease therapies .
Production and Purification Workflow
The recombinant protein is generated via:
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Cloning: Full-length MT-ND4L gene insertion into E. coli vectors .
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Expression: Induced under optimized bacterial culture conditions .
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Purification: Affinity chromatography (His-tag) followed by lyophilization .
| Step | Key Parameters |
|---|---|
| Lyophilization | Trehalose preserves protein stability |
| Storage | -20°C/-80°C; avoid freeze-thaw cycles |
Clinical and Evolutionary Relevance
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LHON Association: The Val65Ala mutation disrupts proton translocation in humans, though mechanistic details remain unresolved .
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Species-Specific Features: Microcebus griseorufus MT-ND4L provides insights into primate mitochondrial evolution and adaptation .
Limitations and Future Directions
Product Specs
Please note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, kindly indicate them during order placement, and we will fulfill your request.
Note: All our proteins are shipped with standard blue ice packs by default. If dry ice shipping is required, please inform us in advance, as additional fees will apply.
