Recombinant Mormopterus kalinowskii Cytochrome b (MT-CYB)
Description
Functional Role in Mitochondria
MT-CYB is the core subunit of Complex III (ubiquinol-cytochrome c reductase), a key component of the mitochondrial electron transport chain :
-
Role: Facilitates electron transfer from ubiquinol to cytochrome c, generating a proton gradient for ATP synthesis .
-
Catalytic Domains:
Mutations in MT-CYB (e.g., p.Phe18Leu, p.Asp171Asn) alter drug sensitivity and complex activity, as demonstrated in yeast models of human mtDNA variants . While M. kalinowskii MT-CYB has not been directly studied for functional mutations, its structural homology to human MT-CYB suggests conserved mechanisms .
Disease Modeling
Recombinant MT-CYB enables studies on mitochondrial disorders linked to Complex III dysfunction. For example:
-
Antiviral/Drug Interactions: Atovaquone (antimalarial) binds the Q₀ site, altering ATP production .
-
Mitochondrial Diseases: Mutations in MT-CYB correlate with Complex III deficiency, leading to multi-organ failure .
Evolutionary Studies
-
Phylogenetic Analysis: Cytochrome b sequences are used to trace bat evolution, as seen in Keenocardium buelowi studies .
-
Positive Selection: cob genes in marine species exhibit adaptive evolution, suggesting functional diversification .
Comparative Analysis with Other Bats
Recombinant cytochrome b proteins from related bat species are also available:
| Species | Catalog Number | Expression System |
|---|---|---|
| Eumops glaucinus | CSB-CF654264EBAC | E. coli |
| Lasionycteris noctivagans | CSB-CF654942LBAI | E. coli |
| Tadarida brasiliensis | CSB-CF657773TAI | E. coli |
These proteins share structural homology but differ in application-specific modifications (e.g., His-tag positioning) .
Challenges and Future Directions
Product Specs
Note: We will prioritize shipping the format that we have in stock. However, if you have a specific format requirement, please indicate it when placing your order, and we will prepare accordingly.
Note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please contact us in advance as additional fees will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
